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Multispectral open-air intraoperative fluorescence imaging

机译：多光谱露天术中荧光成像

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摘要

Intraoperative fluorescence imaging informs decisions regarding surgical margins by detecting and localizing signals from fluorescent reporters, labeling targets such as malignant tissues. This guidance reduces the likelihood of undetected malignant tissue remaining after resection, eliminating the need for additional treatment or surgery. The primary challenges in performing open-air intraoperative fluorescence imaging come from the weak intensity of the fluorescence signal in the presence of strong surgical and ambient illumination, and the auto-fluorescence of nontarget components, such as tissue, especially in the visible spectral window (400-650 nm). In this work, a multispectral open-air fluorescence imaging system is presented for translational image-guided intraoperative applications, which overcomes these challenges. The system is capable of imaging weak fluorescence signals with nanomolar sensitivity in the presence of surgical illumination. This is done using synchronized fluorescence excitation and image acquisition with real-time background subtraction. Additionally, the system uses a liquid crystal tunable filter for acquisition of multispectral images that are used to spectrally unmix target fluorescence from non-target auto-fluorescence. Results are validated by preclinical studies on murine models and translational canine oncology models. (C) 2017 Optical Society of America
机译：术中荧光成像通过检测和定位来自荧光报告者的信号，标记靶标在荧光组织如恶性组织的靶标来通知关于手术边缘的决定。该指导降低了切除后剩余未检测到的恶性组织的可能性，消除了需要额外的治疗或手术。进行露天术中荧光成像的主要挑战来自荧光信号在存在强外科和环境照明的情况下的弱强度，以及非易靶案组分的自荧光，例如组织，尤其是在可见光谱窗口中（ 400-650 nm）。在这项工作中，提供了一种多光谱露天荧光成像系统，用于转换图像引导的术中应用，其克服了这些挑战。该系统能够在外科照明存在下以纳米摩尔敏感性成像弱荧光信号。这是使用同步荧光激励和图像采集完成的，利用实时背景减法来完成。另外，该系统使用液晶可调滤波器，用于获取来自非靶自荧光的谱谱谱谱的多光谱图像。结果是通过对小鼠模型和翻译犬肿瘤学模型的临床前研究验证。 （c）2017年光学学会

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