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首页> 外文期刊>Optik: Zeitschrift fur Licht- und Elektronenoptik: = Journal for Light-and Electronoptic >Orthogonal linear polarization subtraction confocal method for lateral resolution enhancement
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Orthogonal linear polarization subtraction confocal method for lateral resolution enhancement

机译:横向分辨率增强的正交线性偏振减法分组方法

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摘要

Confocal microscopy is a valuable tool for modern life science research. Subtraction microscopy can improve the resolution of the confocal system by the simple addition of components, but the image distortion that is produced can be problematic. In this paper, an orthogonal linear polarization subtraction confocal method (OLSCM) is proposed. The OLSCM obtains images using orthogonal linearly polarized illumination separately and obtains the calculated image by subtracting the absolute difference between the images from the arithmetic mean of the images. A resolution improvement of 14.6% without image distortion can be achieved with a pinhole of one airy unit. In addition, we experimented with 60 nm gold particles and achieved a resolution improvement of 10.3%. In the OLSCM, solid-shaped and hollow-shaped point spread functions (PSFs) are both calculated from the PSFs of pairs of orthogonal linearly polarized illuminations. A resolution improvement can be achieved by using the subtraction method without image distortion. (C) 2017 Elsevier GmbH. All rights reserved.
机译:共聚焦显微镜是现代生活科学研究的宝贵工具。减法显微镜可以通过简单地添加组件来改善共聚焦系统的分辨率,但是产生的图像失真可能是有问题的。本文提出了一种正交线性偏振减法共焦方法(OLSCM)。 OLSCM通过单独使用正交线性偏振光照射获得图像,并通过从图像的算术平均值中减去图像之间的绝对差异来获得计算的图像。通过一个通气单元的针孔可以实现14.6%而没有图像失真的分辨率提高。此外,我们实验了60纳米金颗粒,并达到了10.3%的分辨率提高。在OLSCM中,使用正面和空心形点扩频功能(PSF)均由正交线性偏振照明的PSF计算。可以通过使用没有图像失真的减法方法来实现分辨率改进。 (c)2017年Elsevier GmbH。版权所有。

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