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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >A comparative study of two analytical techniques for the simultaneous determination of amprolium HCl and ethopabate from combined dosage form and in presence of their alkaline degradation
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A comparative study of two analytical techniques for the simultaneous determination of amprolium HCl and ethopabate from combined dosage form and in presence of their alkaline degradation

机译：两种分析技术与组合剂型同时测定氨基HCl和乙酸族的两种分析技术的对比研究及其碱性降解存在

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Chemometric and separation-based techniques (HPLC) are the most applicable and versatile analytical techniques for the analysis of multicomponent mixtures, in the present contribution, a comparison was highlighted between the two analytical techniques of utmost importance as stability indicating assays: UV-spectrophotometry and HPLC-UV focusing on the greenness of each for the simultaneous determination of amprolium HCl (AMP) and ethopabate (ETHOP) in the presence of their alkaline degradation products. The first method was chemometric methods applied were PIS-1, GA-PIS and GA-ANN. To compare the prediction ability of the models, a 4-factor 5-level experimental design was used to establish a calibration set of 25 mixtures containing different ratios of the drugs and their degradation products. The validity of the proposed methods was assessed using an independent validation set of 5 mixtures. The comparison between the different models showed the superiority of ANN model in solving the highly overlapped spectra of the quatemary mixture, yet using inexpensive and easy to handle instruments like the UV-VIS spectrophotometer. The ANN method was used for the quantitative analysis of the drugs in pharmaceutical dosage form via handling the UV spectral data. The second method was based on liquid chromatographic HPLC determination of AMP and ETHOP using C18 column (250 x 4.6 mm(2))-PRONTOSIL 5 mu m, a mobile phase consisting of methanol: Hexane sulphonic acid sodium salt at (pH 3.4 +/- 0.2) adjusted by orthophosphoric add (55:45 v/v). Quantitation was achieved with UV detection at 270 nm at temperature 24 degrees C. Linearity, accuracy and precision were found to be acceptable over the concentration range of 10.0-70.0 and 1.0-25.0 mu g . mL(-1) for AMP and ETHOP, respectively. The proposed methods could be successfully applied for the routine analysis of the studied drugs either in their pure bulk powders or in their pharmaceutical preparations without any preliminary separation step. The results obtained were statistically compared with those obtained by applying the reported method. (C) 2020 Elsevier B.V. All rights reserved.
机译：基于化学计量和分离的技术（HPLC）是用于分析多组分混合物的最适用和通畅的分析技术，在目前的贡献中，在最重要的两种分析技术中突出了比较，作为稳定性指示测定：UV-分光光度法和HPLC-UV聚焦各自用于同时测定其碱性降解产物存在下氨基HCl（AMP）和乙丙酯（ethop）。第一种方法是施用的化学计量方法是PIS-1，GA-PIS和GA-ANN。为了比较模型的预测能力，使用4因素5级实验设计来建立含有不同比例的25个混合物的校准组及其降解产物。使用5个混合物的独立验证组评估所提出的方法的有效性。不同模型之间的比较显示了ANN模型在求解额外混合物的高度重叠光谱时的优越性，但使用廉价且易于处理紫外 - VIS分光光度计等仪器。 ANN方法用于通过处理UV光谱数据来用于药物剂型中药物的定量分析。第二种方法基于液相色谱HPLC使用C18柱测定AMP和ethop（250×4.6mm（2）） - Prontosil5μm，由甲醇：己烷磺酸钠盐（pH3.4 + / /）组成的流动相 - 0.2）通过正磷酸酯调整（55:45 v / v）。通过UV检测在270nm处在温度24℃下进行定量，发现线性，精度和精度在10.0-70.0和1.0-25.0μg的浓度范围内可接受。分别为AMP和ethop的ml（-1）。所提出的方法可以成功地应用于其纯散粉或药物制剂中所研究的药物的常规分析，而无需任何初步分离步骤。与通过施加报告的方法获得的结果进行统计学地进行统计学。 （c）2020 Elsevier B.v.保留所有权利。

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