• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Surgical Endoscopy >Confocal laser microscopy as novel approach for real-time and in-vivo tissue examination during minimal-invasive surgery in colon cancer
【24h】

Confocal laser microscopy as novel approach for real-time and in-vivo tissue examination during minimal-invasive surgery in colon cancer

机译：共聚焦激光显微镜作为结肠癌微创手术期间实时和体内组织检查的新方法

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BackgroundHistological analysis of surgical specimen is the gold standard for cancer classification. In particular, frozen histological diagnosis of vague peritoneal spots or uncertain excision of tumors plays a crucial role in the decision to proceed with or abandon an operation. Confocal laser microscopy (CLM) enables in-vivo and real-time high-resolution tissue analysis. This method has already been used during endoscopic assessments analyzing transformation of esophageal or colon mucosa. We examined whether a CLM device enables to distinguish between non-malignant and malignant tissue in vivo and real time and enables to assign peritoneal carcinomatosis spots to their primary tumor. In addition, we investigated whether the newly developed CLM camera device causes any tissue damage.MethodsCC531 colon carcinoma cells were implanted on the serosa side of the colon and intraperitoneally in Wag/Rija rats via laparotomy. After 7days of tumor growth, confocal laser microscopy in vivo was performed by re-laparotomy. Images of non-malignant and malignant tissue were characterized in terms of specific signal pattern. No fluorescent dye was used. Correlations to findings in conventional histology were systematically recorded and described. Potential tissue damage was examined by conventional histology.ResultsAll animals survived the operative procedure and could be evaluated 7days following surgery. No unexpected death occurred after surgery. Non-malignant colon is defined by small cycles of the microvilli of the colon. There is repetitive deregulated structure in colon carcinoma. Peritoneal carcinomatosis showed the same structural pattern as in primary colon carcinoma. In all examined cases, it was possible to differentiate between peritoneal carcinomatosis spots and non-malignant peritoneum. The CLM device did not cause any tissue damage.ConclusionsThe CLM camera device reported here is feasible to identify peritoneal carcinomatosis spots, assign these spots to the primary tumor, as well as distinguish between non-malignant and malignant tissue in without using any fluorescent dye.
机译：外科标本的背景分析是癌症分类的金标准。特别是，冷冻组织学诊断模糊腹膜斑点或不确定的肿瘤切除在决定继续或放弃操作中起着至关重要的作用。共聚焦激光显微镜（CLM）使体内和实时高分辨率组织分析能够实现。在内窥镜评估期间已经使用该方法分析食管或结肠粘膜的转化。我们检查了CLM器件是否能够在体内和实时区分非恶性和恶性组织，并且能够将腹膜癌症分配给其原发性肿瘤。此外，我们研究了新开发的CLM摄像机装置是否导致任何组织损伤..在通过剖腹手术术通过腹腔切开术植入结肠癌的血清侧和腹膜内植入结肠癌侧的血清癌细胞。在肿瘤生长7天后，通过重新剖腹术进行体内的共聚焦激光显微镜。在特定信号模式方面表征了非恶性肿瘤组织的图像。没有使用荧光染料。系统地记录和描述了与常规组织学中的结果相关的相关性。通过常规组织学检查潜在的组织损伤。培养物动物幸存下来，可以在手术后7天进行评估。手术后没有意外死亡。非恶性结肠由结肠微毛虫的小循环定义。结肠癌有重复的解除管制结构。腹膜癌症显示出与原代结肠癌相同的结构图案。在所有检查的病例中，可以区分腹膜癌症斑点和非恶性腹膜。 CLM器件没有引起任何组织损伤。在此报告的CLM摄像机器件可行的是识别腹膜癌斑点，将这些斑点分配给原发性肿瘤，以及区分非恶性肿瘤在不使用任何荧光染料的情况下。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号