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首页> 外文期刊>Current Microbiology: An International Journal >Characterization of Single Spore Isolates of Agaricus bisporus (Lange) Imbach Using Conventional and Molecular Methods
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Characterization of Single Spore Isolates of Agaricus bisporus (Lange) Imbach Using Conventional and Molecular Methods

机译：使用常规和分子方法表征双孢蘑菇（Lange）Imbach的单个孢子分离物

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摘要

Strains A-15, S11, S-140, and U3 of Agaricus bisporus (Lange) Imbach, were used as parent strains for raising single spore homokaryotic isolates. Out of total 1,642 single spore isolates, only 36 single spore isolates were homokaryons and exhibited slow mycelial growth rate (a parts per thousand currency sign2.0 mm/day) and appressed colony morphology. All these SSIs failed to produce pinheads in Petri plates even after 65 days of incubation, whereas the strandy slow growing SSIs along with parent strains were able to form the fructification in petriplates after 30 days. Out of 24, six ISSR primers, exhibited scorable bands. In the ISSR fingerprints, single spore isolates, homokaryons, lacked amplification products at multiple loci; they grow slowly and all of them had appressed types of colony morphology. The study revealed losses of ISSR polymorphic patterns in non-fertile homokaryotic single spore isolates compared to the parental control or fertile heterokaryotic single spore isolates.
机译：双孢蘑菇（Lange）Imbach的菌株A-15，S11，S-140和U3被用作亲本菌株，用于培养单孢子纯核分离株。在总共1,642个单孢子分离株中，只有36个单孢子分离株是同核体，其菌丝生长速度较慢（每千个货币符号的一部分为2.0毫米/天），并具有明显的菌落形态。即使经过65天的培养，所有这些SSI都无法在陪替氏培养皿中产生钉头状，而缓慢增长的股状SSI与亲本菌株一起能够在30天后在陪替氏培养皿中形成果实。在24种中，有6种ISSR引物显示出可评分的条带。在ISSR指纹图中，单孢子分离株，同核体在多个基因座上缺乏扩增产物。它们生长缓慢，并且所有人都有适当的菌落形态。该研究表明，与亲本对照或可育异核单孢子分离株相比，非可育同核单孢子分离株中ISSR多态性模式的丧失。

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