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首页> 外文期刊>Current Microbiology: An International Journal >Pseudomonas oleovorans subsp. lubricantis subsp. nov., and Reclassification of Pseudomonas pseudoalcaligenes ATCC 17440T as Later Synonym of Pseudomonas oleovorans ATCC 8062T
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Pseudomonas oleovorans subsp. lubricantis subsp. nov., and Reclassification of Pseudomonas pseudoalcaligenes ATCC 17440T as Later Synonym of Pseudomonas oleovorans ATCC 8062T

机译：假单胞菌亚种。润滑剂亚种十月十一日，和假性假单胞菌ATCC 17440T的重新分类，作为油假单胞菌ATCC 8062T的后来同义词

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Isolate RS1T isolated from used metalworking fluid was found to be a Gram-negative, motile, and non-spore forming rod. Based on phylogenetic analyses with 16S rRNA, isolate RS1T was placed into the mendocina sublineage of Pseudomonas. The major whole cell fatty acids were C:s7c (32.6%), C: (25.5%), and C: ISO 2OH/C:s7c (14.4%). The sequence similarities of isolate RS1T based on gyrB and rpoD genes were 98.9 and 98.0% with Pseudomonas pseudoalcaligenes, and 98.5 and 98.1% with Pseudomonas oleovorans, respectively. The ribotyping pattern showed a 0.60 similarity with P. oleovorans ATCC 8062T and 0.63 with P. pseudoalcaligenes ATCC17440T. The DNA G + C content of isolate RS1T was 62.2 mol.%. The DNA-DNA relatedness was 73.0% with P. oleovorans ATCC 8062T and 79.1% with P. pseudoalcaligenes ATCC 17440T. On the basis of morphological, biochemical, and molecular studies, isolate RS1T is considered to represent a new subspecies of P. oleovorans. Furthermore, based on the DNA-DNA relatedness (>70%), chemotaxonomic, and molecular profile, P. pseudoalcaligenes ATCC 17440T and P. oleovorans ATCC 8062T should be united under the same name; according to the rules of priority, P. oleovorans, the first described species, is the earlier synonym and P. pseudoalcaligenes is the later synonym. As a consequence, the division of the species P. oleovorans into two novel subspecies is proposed: P. oleovorans subsp. oleovorans subsp. nov. (type strain ATCC 8062T = DSM 1045T = NCIB 6576T), P. oleovorans subsp. lubricantis subsp. nov. (type strain RS1T = ATCC BAA-1494T = DSM 21016T).
机译：发现从用过的金属加工液中分离出的分离物RS1T是革兰氏阴性，活动性和非孢子形成棒。根据16S rRNA的系统发育分析，将分离的RS1T置于假单胞菌的月经过中。主要的全细胞脂肪酸是C：s7c（32.6％），C：（25.5％）和C：ISO 2OH / C：s7c（14.4％）。基于gyrB和rpoD基因的分离株RS1T的序列相似性与假拟假单胞菌基因分别为98.9％和98.0％，与嗜油假单胞菌分别为98.5％和98.1％。核糖型分型显示与油橄榄假单胞菌ATCC 8062T的相似度为0.60，与假拟疟原虫ATCC17440T的相似度为0.63。分离物RS1T的DNA G + C含量为62.2mol。％。食油假单胞菌ATCC 8062T的DNA-DNA相关性为73.0％，假产假杆状假单胞菌ATCC 17440T的DNA-DNA相关性为79.1％。根据形态学，生化和分子学研究，分离株RS1T被认为代表了新的oleovorans亚种。此外，根据DNA-DNA的相关性（> 70％），化学分类学和分子谱，假拟青假单胞菌ATCC 17440T和油橄榄假单胞菌ATCC 8062T应以相同的名称联合;根据优先权规则，最早描述的物种油橄榄（P. oleovorans）是较早的同义词，而拟假丝藻（P. pseudoalcaligenes）是较晚的同义词。结果，提出了将油橄榄假单胞菌物种分为两个新的亚种：油橄榄假单胞菌。油菜亚种。十一月（菌株ATCC 8062T = DSM 1045T = NCIB 6576T），油橄​​榄假单胞菌。润滑剂亚种十一月（型号RS1T = ATCC BAA-1494T = DSM 21016T）。

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