...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Physics in medicine and biology. >Three-dimensional labeling of newly formed bone using synchrotron radiation barium K-edge subtraction imaging
【24h】

Three-dimensional labeling of newly formed bone using synchrotron radiation barium K-edge subtraction imaging

机译：使用同步辐射钡k缘减法成像的新形成骨的三维标记

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bone is a dynamic tissue which exhibits complex patterns of growth as well as continuous internal turnover (i.e. remodeling). Tracking such changes can be challenging and thus a high resolution imaging-based tracer would provide a powerful new perspective on bone tissue dynamics. This is, particularly so if such a tracer can be detected in 3D. Previously, strontium has been demonstrated to be an effective tracer which can be detected by synchrotron-based dual energy K-edge subtraction (KES) imaging in either 2D or 3D. The use of strontium is, however, limited to very small sample thicknesses due to its low K-edge energy (16.105 keV) and thus is not suitable for in vivo application. Here we establish proof-of-principle for the use of barium as an alternative tracer with a higher K-edge energy (37.441 keV), albeit for ex vivo imaging at the moment, which enables application in larger specimens and has the potential to be developed for in vivo imaging of preclinical animal models. New bone formation within growing rats in 2D and 3D was demonstrated at the Biomedical Imaging and Therapy bending magnet (BMIT-BM) beamline of the Canadian Light Source synchrotron. Comparative x-ray fluorescence imaging confirmed those patterns of uptake detected by KES. This initial work provides a platform for the further development of this tracer and its exploration of applications for in vivo development.
机译：骨是一种动态组织，其表现出复杂的生长模式以及连续内部周转（即重塑）。跟踪此类更改可能是具有挑战性的，因此高分辨率的成像的示踪剂将为骨组织动力学提供强大的新视角。这是，特别是如果可以在3D中检测到这样的示踪剂。以前，已经证明锶是一种有效示踪剂，其可以通过2D或3D的同步基于基于同步的双能k边缘减法（KES）成像来检测。然而，由于其低k边缘能量（16.105keV），锶的使用限制为非常小的样品厚度，因此不适合于体内应用。在这里，我们建立了使用钡作为替代示踪剂的原理原理，具有较高的K-Edge能量（37.441keV），尽管目前用于exvivo成像，这使得能够在较大的标本中施加并且具有潜力开发用于临床前动物模型的体内成像。在二维和三维生长的大鼠内新骨形成的生物医学成像和治疗弯曲磁体加拿大光源同步加速器的（BMIT-BM）束线被证明。对比X射线荧光成像证实了KES检测到的那些摄取模式。这项初步工作为进一步发展此类示踪剂以及对体内发展申请的进一步发展，提供了一个平台。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号