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首页> 外文期刊>Physical chemistry chemical physics: PCCP >The effect of the buffer solution on the adsorption and stability of horse heart myoglobin on commercial mesoporous titanium dioxide: a matter of the right choice
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The effect of the buffer solution on the adsorption and stability of horse heart myoglobin on commercial mesoporous titanium dioxide: a matter of the right choice

机译：缓冲溶液对商业介孔二氧化亚钛马心脏肌红蛋白的吸附和稳定性的影响：正确选择的问题

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摘要

Despite the numerous studies on the adsorption of different proteins onto mesoporous titanium dioxide and indications on the important role of buffer solutions in bioactivity, a systematic study on the impact of the buffer on the protein incorporation into porous substrates is still lacking. We here studied the interaction between a commercial mesoporous TiO2 and three of the most used buffers for protein incorporation, i.e. HEPES, Tris and phosphate buffer. In addition, this paper analyzes the adsorption of horse heart myoglobin (hhMb) onto commercial mesoporous TiO2 as a model system to test the influence of buffers on the protein incorporation behavior in mesoporous TiO2. N-2 sorption analysis, FT-IR and TGA/DTG measurements were used to evaluate the interaction between the buffers and the TiO2 surface, and the effect of such an interaction on hhMb adsorption. Cyclic voltammetry (CV) and electron paramagnetic resonance (EPR) were used to detect changes in the microenvironment surrounding the heme. The three buffers show a completely different interaction with the TiO2 surface, which drastically affects the adsorption of myoglobin as well as its structure and electrochemical activity. Therefore, special attention is required while choosing the buffer medium to avoid misguided evaluation of protein adsorption on mesoporous TiO2.
机译：尽管有许多关于将不同蛋白质的吸附到中孔二氧化钛上的研究以及对生物活性溶液中的重要作用的指示，但对缓冲液对蛋白质掺入到多孔基材中的影响的系统研究仍然缺乏。我们在这里研究了商业介孔TiO2和三种最常用的蛋白质掺入缓冲液之间的相互作用，即HEPES，TRIS和磷酸盐缓冲液。此外，本文分析了马心脏肌球蛋白（HHMB）对商业中孔TiO2作为模型系统，以测试缓冲剂对介孔TiO2中蛋白质掺入行为的影响。使用N-2吸附分析，FT-IR和TGA / DTG测量用于评估缓冲剂和TiO2表面之间的相互作用，以及这种相互作用对HHMB吸附的影响。循环伏安法（CV）和电子顺磁共振（EPR）用于检测血红素周围的微环境的变化。三种缓冲液显示出与TiO 2表面完全不同的相互作用，这大幅影响肌红蛋白的吸附以及其结构和电化学活性。因此，选择缓冲介质的同时需要特别注意，以避免误导评估蛋白质吸附在中孔TiO2上。

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