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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >In vitro micropropagation of Bambusa balcooa Roxb. through nodal explants from field-grown culms and scope for upscaling
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In vitro micropropagation of Bambusa balcooa Roxb. through nodal explants from field-grown culms and scope for upscaling

机译：Bambusa balcooa Roxb的体外微繁殖。通过田间种植的茎的结外植体和扩大规模的范围

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An efficient and reproducible in vitro procedure for large-scale multiplication of Bambusa balcooa Roxb. has been described. Multiple shoot formation (8-10) was observed from excised tender node (12-18 mm in length) containing axillary bud isolated from seconddary branches of 11/2-yr-old culms, when implanted on Murashige and Skoog (MS) medium containing 6-benzylaminopurine (BAP, 1.0 mg l(-1)). Continuous shoot proliferation, tenfold, every 4 weeks was achieved by sub-culturing shoot clumps (2-3 shoots/cluster) in BAP (1.0 mg l(-1)) fortified medium. Seventy-five per cent of shoots could be rooted efficiently on excised propagules when transferred to MS medium supplemented with BAP (1.0 mg l(-1)) and naphthaleneacetic acid (3.0 mg/l). Using this protocol, the first batch of plantlets can be obtained after six months from the initial establishment and then every month new batches of plantlets can be regenerated, depending upon the availability of efficient shoots. After hardening, rooted plantlets were successfully transferred to the soil in polythene sleeves with over 90% survivability and recorded normal growth. Macroproliferation can be done after a period of 5 months from the soil transfer, by which propagules can be increased by more than three times. The tech-economic viability was studied with consideration that the production will be executed in a well-established plant tissue culture laboratory. Factors for characterization at various stages for tissue culture work of B. balcooa have also been studied and evaluated.
机译：一种有效且可重现的体外程序，用于Bambusa balcooa Roxb的大规模繁殖。已经描述过了。当植入含有Murashige和Skoog（MS）的培养基中时，从切除的嫩节（长12-18 mm）中观察到多次芽形成（长度为12-18 mm），其中含有从11/2岁茎的二级分支分离的腋芽6-苄氨基嘌呤（BAP，1.0 mg l（-1））。通过在BAP（1.0 mg l（-1））强化培养基中继代培养枝块（2-3枝/簇），可以实现每4周10倍的连续枝增殖。当转移到补充有BAP（1.0 mg l（-1））和萘乙酸（3.0 mg / l）的MS培养基上时，百分之七十五的芽可以有效地扎根在繁殖的繁殖体上。使用此协议，可以从最初建立六个月后获得第一批苗，然后每个月可以再生新一批苗，具体取决于有效芽的可用性。硬化后，有根小植株成功地以90％以上的存活率转移到聚乙烯套管中的土壤中，并记录了正常的生长。从土壤转移开始，经过5个月的时间即可进行大量增殖，从而使繁殖体增加三倍以上。考虑到将在完善的植物组织培养实验室进行生产，对技术经济可行性进行了研究。还研究和评估了B. balcooa组织培养工作各个阶段表征的因素。

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