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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >DNA microarray analysis targeting pmoA gene reveals diverse community of methanotrophs in the rhizosphere of tropical rice soils
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DNA microarray analysis targeting pmoA gene reveals diverse community of methanotrophs in the rhizosphere of tropical rice soils

机译：靶向pmoA基因的DNA芯片分析揭示了热带稻田土壤根际的甲烷营养菌群落

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摘要

The diversity of the methanotrophs community of two different rice fields of a typical tropical rice agroecosystem was assessed using microarray targeting pmoA gene-based approach. The presence of types I and II methanotrophs was observed with the dominance of Methylocystis in both the fields. The study revealed that the Barkachcha rice field harbours more diverse groups of methanotrophs than the Ghazipur rice field. It was also observed that in some members of types I and II methanotrophs, even the peat-associated group was present in the enriched culture of the soils. The Ghazipur soil and its enriched mixed methanotrophic culture showed higher methane oxidation potential than the Barkachcha soil. These results suggest that the methanotrophs community and its potential for methane oxidation vary with change in soil type within the same ecosystem.
机译：使用靶向pmoA基因的基于基因芯片的方法评估了典型热带稻农业生态系统的两个不同稻田的甲烷营养菌群落的多样性。在两个领域中均观察到以甲基囊藻为主的I型和II型甲烷营养生物。研究表明，比起拉齐普尔稻田，巴尔卡恰稻田所藏的甲烷营养菌群更为多样。还观察到，在I型和II型甲烷营养生物的某些成员中，即使是泥炭相关的基团也存在于土壤的富营养培养中。 Ghazipur土壤及其丰富的混合甲烷营养培养物显示出比Barkachcha土壤更高的甲烷氧化潜力。这些结果表明，在同一生态系统中，甲烷营养生物群落及其甲烷氧化潜力随土壤类型的变化而变化。

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