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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Free diterpenes cafestol and kahweol in beans and in vitro cultures of Coffea species
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Free diterpenes cafestol and kahweol in beans and in vitro cultures of Coffea species

机译：豆类和咖啡因物种的体外培养中的游离二萜类咖啡因和卡哇尔醇

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摘要

Caffeine and chlorogenic acids are the major bioactive metabolites in beans of coffee, which also contains the unique diterpene compounds cafestol and kahweol known to be responsible for hypercholesterolemic effects in humans. The aim of the study was to profile cafestol and kahweol in different plant parts of Coffea species and its beans grown in India, and also in in vitro raised tissues of commercial Indian species, viz. Coffea arabica and Coffea canephora. Total quantities of free diterpene levels were found to be comparatively higher in beans of C. arabica (1.89 mg g(-1)) than in C. canephora (1.13 mg g(-1)). Other plant parts, except the roots, showed considerable amount of cafestol and kahweol. These diterpenes were also synthesized in somatic embryos and in vitro regenerated plants of both Coffea species. Somatic embryos of C. canephora and C. arabica subjected to different hormonal treatments showed variation in the levels of total diterpenes. In this study, we were able to profile cafestol and kahweol levels in in vitro tissues and also in flowers and zygotic embryos of Coffea species. The diterpene profiling in in vitro tissues would be of relevance in future to obtain somaclonal variants with their reduced levels.
机译：咖啡因和绿原酸是咖啡豆中的主要生物活性代谢产物，还含有独特的二萜化合物cafestol和kahweol，已知它们对人的高胆固醇血症有影响。该研究的目的是在印度种植的Coffea品种及其咖啡豆的不同植物部位以及在商业饲养的印度品种的体外培养的组织中，分析咖啡因和kahweol。阿拉伯咖啡和咖啡。发现阿拉比卡角豆中的游离二萜水平总量相对较高（1.89 mg g（-1）），比canephora角豆中（1.13 mg g（-1））高。除根部外，其他植物部位均显示出相当数量的咖啡因和卡威醇。这些二萜还在两种咖啡属的体细胞胚和体外再生植物中合成。接受不同激素处理的C. canephora和C. arabica的体细胞胚显示出总二萜水平的变化。在这项研究中，我们能够剖析咖啡因物种中体外组织，花和合子胚中咖啡因和卡氏甜醇的含量。将来在体外组织中进行二萜谱分析对于获得水平降低的体细胞克隆变异体将具有重要意义。

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