In situ detection of phytoplasma in spike-disease-affected sandal using DAPI stain.

Sunil Thomas; Balasundaran M

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In situ detection of phytoplasma in spike-disease-affected sandal using DAPI stain.

机译：使用DAPI染色剂原位检测受穗病影响的凉鞋中的植物原质。

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Free-hand sections of spike-disease-affected sandal (Santalum album) and its hosts were stained with the DNA-binding fluorochrome, 4,6,diamidino-2-phenyl indole (DAPI), to detect the presence of phytoplasma. Yellow-green fluorescence was detected inthe phloem of diseased sandal, while the phloem tissue of healthy sandal and host plants growing close to the spike-disease-affected sandal in the field as well as in the glasshouse did not show fluorescence. The intensity of fluorescence was high in the young stem and inner bark compared with the root, petiole and leaf. DAPI was more efficient in phytoplasma detection compared with Dienes' stain.

机译：用DNA结合的荧光染料4,6，diamidino-2-phenyl indole（DAPI）染色感染棘突疾病的凉鞋（Santalum album）及其宿主的徒手部分，以检测植物浆体的存在。在患病的凉鞋的韧皮部中检测到黄绿色荧光，而健康的凉鞋和寄主植物的韧皮部组织在田间以及温室附近生长在穗病病害的凉鞋附近，并没有显示荧光。与根，叶柄和叶相比，幼茎和内皮的荧光强度较高。与Dienes染色相比，DAPI在植物质检中更有效。

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《Current Science: A Fortnightly Journal of Research》 |1998年第11期|共5页
作者
Sunil Thomas; Balasundaran M;
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正文语种 eng
中图分类自然科学现状及发展;
关键词
techniques; detection; bark; fluorescence; host plants; hosts; phloem; stains; staining; forest trees; broadleaves; trees; plant pathology; Santalum album; plant viruses;

机译：技术;检测;树皮;荧光;寄主植物;寄主;韧皮部;污点;染色;林木;阔叶树;树木;植物病理学;钽相册;植物病毒;

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1. In situ detection of phytoplasma in spike-disease-affected sandal using DAPI stain. [J] . Sunil Thomas, Balasundaran M Current Science: A Fortnightly Journal of Research . 1998,第11期

机译：使用DAPI染色剂原位检测受穗病影响的凉鞋中的植物原质。
2. Efficacy of nested-PCR for the detection of phytoplasma causing spike disease of sandal [J] . J. A. Khan, P. Srivastava, S. K. Singh Current Science: A Fortnightly Journal of Research . 2004,第11期

机译：巢式PCR检测引起凉鞋穗病的植物原质的功效
3. Efficacy of nested-PCR for the detection of phytoplasma causing spike disease of sandal [J] . Khan J. A., Singh S. K., Srivastava P. Current science . 2004,第11期

机译：巢式PCR检测引起凉鞋穗病的植物原质的功效
4. Combined FISH, Anti-γ-Hb and DAPI for Detection of Fetal Nucleated RBCs in Maternal Blood [C] . Mona Farhad, Jeffrey H. Price Conference on Optical Diagnostics of Living Cells Ⅴ, Jan 23-25, 2002, San Jose, USA . 2002

机译：结合FISH，抗γ-Hb和DAPI检测母体血液中的胎儿有核红细胞
5. Molecular detection and identification of phytoplasmas and establishment of phytoplasma-free clonal plants. [D] . Wang, Keri. 1997

机译：植原体的分子检测和鉴定以及无植原体克隆植物的建立。
6. New Insights into the in situ Microscopic Visualization and Quantification of Inorganic Polyphosphate Stores by 4’6-Diamidino-2-Phenylindole (DAPI)-Staining [O] . F.M. Gomes, I.B. Ramos, C. Wendt, 2013

机译：通过46-Diamidino-2-Phenylindole（DAPI）-染色对无机多磷酸盐存储原位显微图像化和定量化的新见解
7. Cloning and Characterization of the Antigenic Membrane Protein (Amp) Gene and In Situ Detection of Amp from Malformed Flowers Infected with Japanese Hydrangea Phyllody Phytoplasma [O] . R. Arashida, S. Kakizawa, Y. Ishii, 2008

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8. Explosive Residue Detection by Laser Surface Photo-Fragmentation- Fragment Detection Spectroscopy. 2. In Situ and Real-Time Monitoring of RDX. HMX, CL20, and TNT, by an Improved Ion Probe [R] . Cabalo, J. B. , Sausa, R. C. 2005

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In situ detection of phytoplasma in spike-disease-affected sandal using DAPI stain.

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