...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Current Science: A Fortnightly Journal of Research >APPLICATION OF RAPD FINGERPRINTING IN SELECTION OF MICROPROPAGATED PLANTS OF PIPER LONGUM FOR CONSERVATION
【24h】

APPLICATION OF RAPD FINGERPRINTING IN SELECTION OF MICROPROPAGATED PLANTS OF PIPER LONGUM FOR CONSERVATION

机译：RAPD指纹图谱在ONG茎微繁殖植物选择中的应用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Random amplified polymorphic DNA fingerprints of twenty micropropagated plants and the mother plant were analysed by polymerase chain reaction of genomic DNA using ten random 10-mer primers. The RAPD fragments were scored for presence/absence to calculate Jaccard's similarity index. Clustering based on similarity index was done following unweighted pair group with arithmetic mean method and a dendrogram was constructed. The dendrogram showed eighteen micropropagated plants forming a major cluster along with the mother plant. The other two micropropagated plants could be regarded as molecular off-types (putative somaclonal variants) as they have shown less than 80% similarity to the mother plant and other micropropagated plants. Among the eighteen micropropagated plants of the major cluster the order of preference to maintain maximum fidelity to the elite genotype (the mother plant) for conservation was established. [References: 13]
机译：使用十个随机的10-mer引物，通过基因组DNA的聚合酶链反应，分析了20种微繁植物和母本植物的随机扩增多态性DNA指纹。对RAPD片段的存在/不存在进行评分，以计算Jaccard的相似性指数。采用算术平均法对未加权对组进行相似度聚类，构建树状图。树状图显示十八种微繁殖的植物与母本植物一起形成一个主要簇。其他两种微繁殖植物可被视为分子异型（假定为体细胞克隆变异体），因为它们与母本植物和其他微繁殖植物的相似性不到80％。在主要集群的18种微繁殖植物中，建立了优先顺序以保持对保育精英基因型（母本植物）的最大保真度。 [参考：13]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号