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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >A novel haloarchaeal lineage widely distributed in the hypersaline marshy environment of Little and Great Rann of Kutch in India
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A novel haloarchaeal lineage widely distributed in the hypersaline marshy environment of Little and Great Rann of Kutch in India

机译：在印度库奇利特尔和大兰恩的高盐湿地环境中广泛分布的一种新颖的古细菌世系

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摘要

Archaea (Archaebacteria) are a phenotypically diverse group of microorganisms distributed mainly in extreme environments throughout the world. To study the community of archaea present in the extreme salty marshes of the Rann of Kutch, Gujarat, Indiaculture-dependent approach was employed for isolating archaea by mimicking extreme hypersaline environment and 25 extreme halophilic archaea were obtained. Phylogenetic analysis revealed the presence of a number of uncultivated and unidentified members of archaea among the isolates present. Phylogenetic analysis involving the sequence data of 16S rRNA of 25 haloarchaeal isolates, keeping Methanospirillium hungatei DSM864 (M60880) as the out-group, also identified a novel lineage of three isolates. The 16S rRNA sequences of the strains 3A1-DGR, H9-DGR and 2ANA-DGR showed less than 93% similarity with the available known type strains and these three strains belong to a distinct novel lineage within the family Halobacteriaceae, near an uncultivated environmental cluster.
机译：古细菌（古细菌）是表型不同的一组微生物，主要分布在全世界的极端环境中。为了研究古吉拉特邦库奇兰郡极端咸湿地中存在的古细菌群落，采用了印度文化依赖的方法，通过模仿极端高盐环境隔离古细菌，并获得了25种极端嗜盐古细菌。系统发育分析表明，在存在的分离物中，存在许多未培养和未鉴定的古细菌成员。系统进化分析涉及25个卤代古细菌分离株的16S rRNA的序列数据，使杭甲基甲烷螺旋菌DSM864（M60880）处于群外，也确定了这三个分离株的新谱系。菌株3A1-DGR，H9-DGR和2ANA-DGR的16S rRNA序列与可得的已知类型菌株的同源性低于93％，这三个菌株属于卤细菌科中一个独特的新世系，靠近一个未经培养的环境群。

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