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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Somatic embryogenesis in 'maggar' bamboo (Dendrocalamus hamiltonii) and field performance of regenerated plants
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Somatic embryogenesis in 'maggar' bamboo (Dendrocalamus hamiltonii) and field performance of regenerated plants

机译：'maggar'竹（Dendrocalamus hamiltonii）的体细胞胚发生和再生植物的田间表现

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摘要

In vitro regeneration through somatic embryogenesis using explants taken from field-grown 10- and 45-year-old elite maggar bamboo (Dendrocalamus hamiltonii) plants has been demonstrated. The concentration of plant growth substances used in the culture medium, and age of the mother plant (MP) were found to significantly influence callus formation, and subsequent embryogenesis. Eighteen months after transfer to soil, in vitro propagated plants of the two age groups, and the corresponding MPs were compared in respect of gas and water vapour exchange rates, related parameters, morphological features and leaf anatomy. The rate of photosynthesis was significantly influenced by the age of the MPs and was found to be higher in the tissue culture (TC)-raised plants; plants (both TC-raised and MPs) of the younger age group performed better than the corresponding plants of the older age group. The same trend was found when the water-use efficiency was taken into consideration. Thus, based on various parameters studied in the present investigation, in vitro propagated and hardened plants were found to be generally comparable with the corresponding mother plants. The in vitro propagation methodology reported here has already been used for mass-scale production of maggar bamboo.
机译：已证明使用从田间生长的10和45年龄的优质蒲竹（Dendrocalamus hamiltonii）植物中提取的外植体通过体细胞胚发生进行体外再生。发现培养基中使用的植物生长物质的浓度和母体植物的年龄（MP）会显着影响愈伤组织的形成和随后的胚发生。转移到土壤后的18个月，比较了两个年龄组的体外繁殖植物和相应的MP，比较了它们之间的气体和水蒸气交换速率，相关参数，形态特征和叶片解剖结构。光合作用的速率受MP年龄的显着影响，在组织培养（TC）植株中发现更高。较年轻年龄组的植物（TC提高和MPs）的表现要好于较老年龄组的相应植物。当考虑到用水效率时，发现了相同的趋势。因此，基于本研究中研究的各种参数，发现体外繁殖和硬化的植物通常与相应的母本植物具有可比性。此处报道的体外繁殖方法已用于大规模生产玛加竹。

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