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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Pollination without emasculation: an efficient method of hybridization in soybean (Glycine max (L.) Merrill).
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Pollination without emasculation: an efficient method of hybridization in soybean (Glycine max (L.) Merrill).

机译：无去雄授粉：在大豆中杂交的有效方法（Glycine max（L.）Merrill）。

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摘要

Normally, the soyabean breeders perform hybridization through manual emasculation (in the evening) followed by pollination (the next day in the morning). Some breeders prefer simultaneous emasculation and pollination. This paper reports an approach that is devoid of emasculation and ensures less damage to the flower buds, and hence more success. The crossing approach was applied to two intra-specific (Glycine max x G. max) crosses of soyabean: DS9712 x PI542044 and DS9814 x PI542044. The percentage of bud survival and development into full-grown pods ranged from 33 to 38% in F1 generation and 22 to 35% in backcross generations. It was significantly higher than the success (11.53-14.72%) obtained through traditional approaches (emasculation followed by pollination). The success of crossing during rabi season was less compared to kharif because of prolonged cooler temperature with shorter day length that leads to the development of more cleistogamous flower. The hybridity of the F1 plants was established through polymorphic simple sequence repeat markers. The number of true F1 hybrid plants was 78% and 72% in the crosses 1 and 2, respectively. This was also significantly higher than the traditional approach with 64% success. The success was more pronounced in the BC1 and BC2 generations. The rate of success varied between crosses 1 and 2, and more success was observed in all the crosses involving DS9712 as the female parent.
机译：通常，大豆育种者通过手动去雄（晚上）然后授粉（第二天早上）进行杂交。一些育种者喜欢同时去雄和授粉。本文报道了一种方法，该方法无需去雄，可确保对花蕾的伤害更少，从而获得更大的成功。杂交方法应用于大豆的两个种内（最大大豆最大杂交）杂交：DS9712 x PI542044和DS9814 x PI542044。 F 1 世代的芽存活和发育为成年豆荚的百分比在F 1 世代中为33％至38％，在回交世代中为22％至35％。它显着高于通过传统方法（去草后授粉）获得的成功率（11.53-14.72％）。在狂犬病季节进行杂交的成功率比kharif少，这是因为长时间的凉爽温度和较短的日照长度导致了更多杂种花的发育。通过多态性简单序列重复标记建立了F 1 植物的杂种优势。在杂交1和2中，真正的F 1 杂种植物的数量分别为78％和72％。这也大大高于传统方法，成功率为64％。在BC 1 和BC 2 世代中，成功更为明显。成功率在杂交1和2之间有所不同，在所有以DS9712为母本的杂交中都观察到成功。

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