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Wheat genome sequencing: a milestone in cereal genomics and its future potential.

机译:小麦基因组测序:谷物基因组学及其未来潜力的里程碑。

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Random shotgun libraries of total DNA and cDNA from Triticum aestivum cv. Chinese Spring (CS42) were sequenced in Roche 454 pyrosequencer using GS FLX titanium and GS FLX + platforms to generate 85 Gb of sequence (220 million reads), equivalent to 5X genome coverage and identified between 94 000 and 96 000 genes. This paper indicates that the wheat genome sequencing (WGS) represents an essential step towards a systematic understanding of biology and engineering the cereal crop for valuable traits. It show that the whole genome sequence of wheat and the availability of thousands of SNPs will inevitably permit the breeders to stride towards identifying novel traits, providing biological knowledge and empowering biodiversity-based breeding.
机译:普通小麦总DNA和cDNA的随机shot弹枪文库。使用GS FLX钛和GS FLX +平台在Roche 454焦测序仪中对中国春(CS42)进行测序,产生85 Gb的序列(2.2亿个读数),相当于5X基因组覆盖率,并鉴定出94 000至96 000个基因。本文表明,小麦基因组测序(WGS)是朝着系统地了解生物学和对谷物作物进行有价值的性状迈出的重要一步。它表明,小麦的全基因组序列和成千上万的SNP的可用性将不可避免地使育种者大步迈向鉴定新性状,提供生物学知识并增强基于生物多样性的育种。

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