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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >The use of RAPD in assessing genetic variability in Andrographis paniculata Nees, a hepatoprotective drug
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The use of RAPD in assessing genetic variability in Andrographis paniculata Nees, a hepatoprotective drug

机译：RAPD在评估穿心莲（一种保护肝脏的药物）的遗传变异中的用途

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摘要

RAPD analysis was done to determine intraspecific variability in Andrographis paniculata, a popular antipyretic and hepatoprotective drug used in traditional medicine in India. The accessions collected from parts of India and south-east Asia on molecular analysis revealed moderate variation within the species. Similarity measurement using UPGMA followed by cluster analysis resulted in 5 major groups based on geographical distribution that generally reflected expected trends between the genotypes. There were also important exceptions like AP-48, an accession from Thailand showing close resemblance to AP-38 collected from Tamil Nadu and AP-29 from Assam significantly diverse from the rest of the native genotypes. The results indicated that RAPD could be effectively used for genetic diversity analysis in wild species of prospective value as it is reliable, rapid and superior to those based on pedigree information.
机译：进行了RAPD分析，以确定穿心莲中的种内变异性，穿心莲是印度传统医学中常用的解热和保肝药物。从印度和东南亚部分地区收集的分子分析结果表明，该物种内部存在中等差异。使用UPGMA进行相似性测量，然后进行聚类分析，基于地理分布得出了5个主要类别，这些类别通常反映了基因型之间的预期趋势。还有一些重要的例外，例如AP-48，这是泰国的登录品，与从泰米尔纳德邦收集的AP-38和从阿萨姆邦收集的AP-29非常相似，与其他天然基因型截然不同。结果表明，RAPD方法可靠，快速，优于基于谱系信息的野生物种，可有效用于具有潜在价值的野生物种的遗传多样性分析。

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