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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Expression analysis of drought-induced genes in wild tomato line (Solanum habrochaites)
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Expression analysis of drought-induced genes in wild tomato line (Solanum habrochaites)

机译：干旱胁迫下野生番茄品系的基因表达分析

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摘要

Many plant genes are regulated in response to abiotic stresses such as drought, high salinity, heat and cold, and their gene products function in stress response and tolerance. The whole process of plant adaptation to these environmental stresses is controlled by orchestration of complex molecular networks. In the present study, eight genes showing significant difference of expression on exposure to artificial drought stress in tomato, were selected from the previously performed microarray experiment. Expression analysis of the genes was done semi-quantitatively as well as quantitatively under artificially imposed drought stress and the results were almost similar to those of microarray experiment. Tissue-specific analysis of the genes, performed on tolerant line, revealed fairly a similar pattern of expression in root, stem and leaf with notable differences in flower, which experienced the least influence of drought. The results confirmed that SlPRP16, SlCYP51-17, SlMCPI19 and SlGDSL20 were downregulated in both the lines with stronger downregulation in sensitive line. SlWRKY4 was downregulated in both the lines with more folds of downregulation in tolerant line. SlEFH12 and SlSNF4-15 were upregulated in tolerant line. SlUSPA9 was upregulated in both the lines with relatively more folds of up regulation in sensitive line.
机译：许多植物基因响应非生物胁迫（例如干旱，高盐度，高温和寒冷）而受到调节，其基因产物在胁迫响应和耐受性中发挥作用。植物适应这些环境胁迫的整个过程由复杂分子网络的编排控制。在本研究中，从先前进行的微阵列实验中选择了八个基因，这些基因在暴露于番茄的人工干旱胁迫下显示出明显的表达差异。在人工施加的干旱胁迫下，对基因的表达进行了半定量和定量分析，结果几乎与微阵列实验相似。在耐性系上进行的基因组织特异性分析显示，根，茎和叶中的表达模式相当相似，但花的显着差异明显，干旱的影响最小。结果证实，两条线中的SlPRP16，SlCYP51-17，SlMCPI19和SlGDSL20均被下调，而敏感线中的下调更强。在两个品系中SlWRKY4均被下调，而在耐受品系中下调更多倍。 SlEFH12和SlSNF4-15在耐受系中被上调。在这两个品系中，SlUSPA9都被上调，而在敏感品系中，上调相对更多。

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