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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Regeneration of late leaf spot-resistant groundnut plants from Cercosporidium personatum culture filtrate-treated callus.
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Regeneration of late leaf spot-resistant groundnut plants from Cercosporidium personatum culture filtrate-treated callus.

机译：从Cercosporidium personatum培养物滤液处理过的愈伤组织中再生晚叶抗斑花生植株。

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摘要

Cotyledon callus cultures of groundnut derived from C. personatum [Mycosphaerella berkeleyi] susceptible and resistant genotypes were exposed to various concentrations of fungal culture filtrate (FCF) of M. berkeleyi. Fresh weight and cell viabilityof calluses were determined after exposure to various concentrations of FCF. Sensitive calluses failed to increase in fresh weight and lost viability after exposure to media containing the FCF, whereas insensitive calluses retained growth and maintainedviability similar to controls that had not been exposed to FCF. Insensitive calluses were selected by culturing on growth medium containing various concentrations of the FCF. Resistant calluses obtained by selection survived 3 subcultures under the sameconditions and were used for plantlet regeneration. Regenerated plants when transferred to soil-sand mixture in plastic cups and subsequently shifted to field conditions, set few viable seeds. Plants of the R2 generation exhibited enhanced resistance toM. berkeleyi.
机译：将源自人参衣原体[Mycosphaerella berkeleyi]易感和抗性基因型的花生的子叶愈伤组织培养物暴露于不同浓度的伯克利曼螺旋菌的真菌培养物滤液（FCF）。暴露于各种浓度的FCF后，测定老茧的鲜重和细胞活力。暴露于含有FCF的培养基后，敏感的老茧不能增加新鲜体重，丧失活力，而与未暴露于FCF的对照相似，不敏感的老茧可以保持生长并保持活力。通过在含有各种浓度的FCF的生长培养基上培养来选择不敏感的愈伤组织。通过选择获得的抗性老茧在相同条件下存活3次亚培养，并用于苗再生。当再生的植物转移到塑料杯中的土壤-沙土混合物中，然后转移到田间条件下时，结出的种子很少。 R2世代的植物表现出增强的对M的抗性。伯克利伊。

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