Microbial DNA extraction from samples of varied origin.

Chaudhuri S R; Pattanayak A K; Thakur A R

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Microbial DNA extraction from samples of varied origin.

机译：从各种来源的样品中提取微生物DNA。

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The impact of four different soil DNA extraction methods on the quantity and quality of isolated community DNA was evaluated using agarose gel electrophoresis, DNA spectrum study and PCR-based 16S ribosomal DNA analysis. The modified direct lysis method was optimized for environmental water samples (wastewater fed-fisheries and raw liquid sewage canal) as well as diagnostic samples like urine from humans, thus opening up a new arena for fast culture-independent detection of causative pathogens in pathological manifestations like urinary tract infections. The same method was independently found to be effective for isolation of genomic DNA from both Gram-positive and Gram-negative bacteria. Thus a uniform method of DNA extraction from environmental samples (different types of soil and water), pathological samples as well as varied kinds of bacteria was obtained..

机译：使用琼脂糖凝胶电泳，DNA光谱研究和基于PCR的16S核糖体DNA分析，评估了四种不同的土壤DNA提取方法对分离的群落DNA的数量和质量的影响。改进的直接裂解方法针对环境水样品（废水捕捞的渔业和原液污水渠）以及人类尿液等诊断性样品进行了优化，从而开辟了一个新的领域，可快速独立于培养物检测病理表现中的病原体像尿路感染。独立地发现相同的方法对于从革兰氏阳性和革兰氏阴性细菌中分离基因组DNA是有效的。因此，获得了从环境样品（不同类型的土壤和水），病理样品以及各种细菌中提取DNA的统一方法。

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《Current Science: A Fortnightly Journal of Research》 |2006年第12期|共4页
作者
Chaudhuri S R; Pattanayak A K; Thakur A R;
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正文语种 eng
中图分类自然科学现状及发展;
关键词
biodiversity; DNA sequencing; extraction; fish scrap; Gram negative bacteria; Gram positive bacteria; human diseases; methodology; microbial flora; nucleotide sequences; pathogens; pathology; public health; ribosomal DNA; sewage; soil flora; so il types; urinary tract infections; urine; waste water;

机译：生物多样性;DNA测序;提取;鱼残;革兰氏阴性细菌;革兰氏阳性细菌;人类疾病;方法;微生物菌群;核苷酸序列;病原体;病理学;公共卫生;核糖体DNA;污水;土壤菌群;特殊类型;泌尿道感染;尿液;废水;

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专利

1. Microbial DNA extraction from samples of varied origin. [J] . Chaudhuri S R, Pattanayak A K, Thakur A R Current Science: A Fortnightly Journal of Research . 2006,第12期

机译：从各种来源的样品中提取微生物DNA。
2. Statistical assessment of DNA extraction methodology for culture-independent analysis of microbial community associated with diverse environmental samples [J] . Mahajan Rishi, Attri Sampan, Sharma Kavita, Molecular biology reports . 2018,第3期

机译：DNA提取方法的统计评估与不同环境样品相关的微生物群体培养型分析
3. OPTIMIZATION OF DNA EXTRACTION FROM HUMAN URINARY SAMPLES FOR MICROBIAL COMMUNITY PROFILING [J] . Ackerman A. Lenore, Eilber Karyn S., Tang Jie, Neurourology and urodynamics. . 2017,第Suppla1期

机译：对人类尿液样品进行微生物群落分析的DNA提取优化
4. Rapid Detection of Microbial Pathogen in Blood Culture Bottles Using Punch-it? NA-Sample DNA Extraction Kit and Reverse Blot Hybridization Assay [C] . Kwangmin Yu, Seoyong Kim, Jungho Kim, International Molecular Medicine Tri-Conference. . 2016

机译：使用冲床快速检测血液培养瓶中的微生物病原体吗？ Na样谱DNA提取试剂盒和逆印杂交测定
5. Filter sampling of airborne microbial agents: Evaluation of filter materials for physical collection efficiency, extraction, and comparison to traditional bioaerosol sampling. [D] . Burton, Nancy Clark. 2007

机译：空气中微生物制剂的过滤采样：评估过滤材料的物理收集效率，提取并与传统生物气溶胶采样进行比较。
6. Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction [O] . Hengameh Mirsepasi, Søren Persson, Carsten Struve, 2014

机译：粪便样品中的微生物多样性取决于DNA提取方法：easyMag DNA提取与QIAamp DNA粪便微型试剂盒提取相比
7. Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction [O] . Hengameh Mirsepasi, Søren Persson, Carsten Struve, 2014

机译：粪便样品中的微生物多样性取决于DNA提取方法：easyMag DNA提取与QIAamp DNA粪便微型试剂盒提取相比
8. Comparison of Three DNA Extraction Kits to Establish Maximum Yield and Quality of Coral-Associated Microbial DNA [R] . Baker, E J, Kellogg, CA 2014

机译：三种DNa提取试剂盒的比较建立珊瑚相关微生物DNa的最大产量和质量

Microbial DNA extraction from samples of varied origin.

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