Development of a single-tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper

Bhat AI; Siju S

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Development of a single-tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper

机译：开发用于同时检测与黑胡椒特技病有关的黄瓜花叶病毒和Piper黄斑驳病毒的单管多重RT-PCR技术

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Single-tube multiplex RT-PCR was developed for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus in black pepper. PCR primers designed for amplifying 650 bp from the coat protein gene of CMV and 450 bp from the open reading frame I of PYMoV were used in the reaction. The quantity of template used in mRT-PCR was found to be crucial for successful amplification. The method was found successful for the detection of both the viruses infecting black pepper in nursery and field samples. The method is rapid, reliable and requires only small tissue sample for sensitive detection. The entire procedure can aid in the rapid screening of a large number of plants for both viruses.

机译：开发了用于同时检测黑胡椒中黄瓜花叶病毒和Piper黄斑驳病毒的单管多重RT-PCR。反应中使用了设计用于从CMV外壳蛋白基因扩增650 bp和从PYMoV的开放阅读框I扩增450 bp的PCR引物。发现用于mRT-PCR的模板数量对于成功扩增至关重要。发现该方法可成功检测出苗圃和田间样品中感染黑胡椒的病毒。该方法快速，可靠，并且仅需少量组织样品即可进行灵敏检测。整个过程可以帮助快速筛选大量植物中的两种病毒。

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《Current Science: A Fortnightly Journal of Research》 |2007年第7期|共3页
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Bhat AI; Siju S;
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中图分类自然科学现状及发展;
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1. Development of a single-tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper [J] . Bhat AI, Siju S Current Science: A Fortnightly Journal of Research . 2007,第7期

机译：开发用于同时检测与黑胡椒特技病有关的黄瓜花叶病毒和Piper黄斑驳病毒的单管多重RT-PCR技术
2. Identification and transmission of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum) in Sri Lanka [J] . D. P. P. de Silva, P. Jones, M. W. Shaw Plant Pathology . 2002,第5期

机译：斯里兰卡感染黑胡椒的胡椒黄斑驳病毒和黄瓜花叶病毒的鉴定和传播
3. Simultaneous detection of wheat dwarf virus, northern cereal mosaic virus, barley yellow striate mosaic virus and rice black-streaked dwarf virus in wheat by multiplex RT-PCR [J] . Journal of Virological Methods . 2017,第期

机译：用多重RT-PCR同时检测小麦矮化病毒，北谷物叶片病毒，大麦黄色条纹叶片病毒和小麦的黑色条纹矮化病毒
4. Risk Assessment Procedure for Cucumber Vein Yellowing Virus (CVYV) and Cucurbit Yellow Stunting Disorder Virus (CYSDV) Using a Real Time RT-PCR Assay - Study of Virus Levels in the Vector Bemisia tabaci [C] . F.M. Gil-Salas, J. Morris, I.M. Cuadradol, International Symposium on Vegetable Production, Quality and Process Standardization in Chain . 2012

机译：使用实时RT-PCR测定的黄瓜静脉燃气病毒（CVYV）和葫芦炎黄色衰退病毒（CYSDV）的风险评估程序 - 载体Bemisia Tabaci中病毒水平的研究
5. Functional investigations of the Lettuce infectious yellow virus (LIYV)-encoded proteins, P34, P5, P9 and the development of LIYV and Cucurbit yellow stunting disorder virus (CYSDV) immunity using RNA interference. [D] . Kiss, Zsofia Agnes. 2013

机译：生菜传染性黄色病毒（LIYV）编码的蛋白质P34，P5，P9的功能研究以及使用RNA干扰的LIYV和葫芦黄色发育不良病毒（CYSDV）免疫力的发展。
6. Development of a multiplex RT-PCR assay for simultaneous detection of Cucumber green mottle mosaic virus and Acidovorax citrulli in watermelon [O] . Xinyue Bi, Xiaodong Li, Haibo Yu, -1

机译：同时检测西瓜中的黄瓜绿斑驳花叶病毒和瓜酸衣藻的多重RT-PCR检测方法的建立
7. Identification and transmission of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum ) in Sri Lanka [O] . D. P. P. De Silva, P. Jones, M. W. Shaw 2002

机译：在斯里兰卡的吹笛剂黄色斑点病毒和黄瓜黄瓜（吹笛者Nigrum）的识别和传输

Development of a single-tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper

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