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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Isolation of predominant bacterium from gut of earthworm Lampito mauritii for effective use in soil fertility
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Isolation of predominant bacterium from gut of earthworm Lampito mauritii for effective use in soil fertility

机译：从maLampito mauritii的肠道中分离出主要细菌以有效地用于土壤肥力

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摘要

Lampito mauritii is an anecic earthworm living in the topsoil and it is geophytophagous in nature. This earthworm is an important soil macrofauna as it has the dual role of an 'ecosystem engineer' due to the ability to build burrows as well as 'keystone species' in soil food webs because of its function in degradation of organic wastes. The present study investigates the gut of this earthworm to find the most predominant bacterium harboured therein. Gut contents were regularly extracted and streaked on bacteriological media. The predominant type of colony was identified, isolated and streaked separately to get pure colonies. The microbe was subjected to several biochemical tests and also 16S rRNA sequencing for identification. On the basis of these tests, the bacterium was identified as Bacillus cereus. The microbe was used as a composting agent on solid wastes as a result of which good amount of plant nutrients, specially nitrogen (20.3 kg/acre), phosphate (27.4 kg/acre) and potassium (52.1 kg/acre) were found in the resultant manure. The compost thus obtained was then utilized for the production of vegetables with an attempt to protect soil environment, thus reducing the deleterious effects of chemical fertilizers.
机译：兰皮托莫里蒂（Lampito mauritii）是生活在表土中的一种食ec，其本质上是食植物的。 earth是重要的土壤大型动物，因为它具有在有机食物中降解的功能，因此能够在土壤食物网中建造洞穴和“关键物种”，因此具有“生态系统工程师”的双重作用。本研究调查了这种earth的肠道，以发现其中蕴藏的最主要的细菌。定期提取肠内容物，并在细菌培养基上划线。鉴定出主要的菌落类型，将其分离并划线以得到纯菌落。对该微生物进行了几次生化测试，还进行了16S rRNA测序以进行鉴定。在这些测试的基础上，该细菌被鉴定为蜡状芽孢杆菌。该微生物被用作固体废物的堆肥剂，其结果是在其中发现了大量的植物养分，特别是氮（20.3千克/英亩），磷酸盐（27.4千克/英亩）和钾（52.1千克/英亩）。产生的肥料。然后将由此获得的堆肥用于蔬菜生产，以保护土壤环境，从而减少化学肥料的有害作用。

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