• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Identification and characterization of a stress-inducible gene OsNLI-IF enhancing drought tolerance in transgenic tobacco
【24h】

Identification and characterization of a stress-inducible gene OsNLI-IF enhancing drought tolerance in transgenic tobacco

机译：胁迫诱导基因OsNLI-IF增强转基因烟草耐旱性的鉴定与鉴定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Plants respond to the adverse environment by activating a series of stress-inducible genes, including genes encoding transcription factors. The expression of these genes is regulated by a core DNA sequence contained in their promoter region called cis-acting element. The promoter region of several stress-responsive genes contains several stress-regulated cis-acting elements such as dehydration-responsive element, Crepeat, low-temperature-responsive element, NAC recognition sequence and ZFHD recognition sequence. In this study we isolated a cDNA for a transcription factor named nuclear LIM interactor-interacting factor from rice cDNA library by yeast one-hybrid screening using two target sequences of 50 nucleotides derived from two stress-inducible promoters, JRC0528 and JRC0332, of cold-inducible genes OsZF1 and OsNAC6 respectively, as baits. The NLI-IF protein showed both DNA-binding and transcriptional activities in yeast experiments. Expression of OsNLI-IF was found to be induced by cold, heat, salt and drought stresses. The OsNLI-IF gene overexpressing transgenic tobacco plants showed improvement in drought tolerance. The present study emphasizes that OsNLI-IF could be useful for development of drought-tolerant transgenic crop plants.
机译：植物通过激活一系列胁迫诱导基因（包括编码转录因子的基因）来应对不利环境。这些基因的表达受其启动子区域中称为顺式作用元件的核心DNA序列的调节。几个应激反应基因的启动子区域包含几个应激调节的顺式作用元件，例如脱水反应元件，绉纹，低温反应元件，NAC识别序列和ZFHD识别序列。在这项研究中，我们通过酵母单杂交筛选从水稻cDNA文库中分离出了一个名为核LIM相互作用因子的转录因子的cDNA，该序列使用两个来自50个核苷酸的靶标序列从两个胁迫诱导型启动子JRC0528和JRC0332进行了冷一诱导基因OsZF1和OsNAC6分别作为诱饵。在酵母实验中，NLI-IF蛋白既显示DNA结合活性，又显示转录活性。发现OsNLI-IF的表达是由冷，热，盐和干旱胁迫诱导的。过表达OsNLI-IF基因的转基因烟草植物显示出对干旱耐受性的改善。本研究强调OsNLI-IF可能对耐旱的转基因作物植物的发展有用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号