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Fine mapping of consistent quantitative trait loci for yield under drought stress using rice (Oryza sativa) recombinant inbred lines adapted to rainfed environment

机译:使用适合雨育环境的水稻重组自交系对干旱胁迫下产量的一致数量性状基因座进行精细定位

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摘要

Drought stress is a serious constraint, especially in rainfed rice production, and breeding for drought tolerance by selection based on yield under stress, though effective, is slow. Mapping quantitative trait loci (QTLs) for yield and its components under drought stress predominant in rainfed target populations of environment (TPE) will help overcome this limitation. In the present study, a subset of 143 F-8 and F-9 recombinant inbred (RI) lines derived from IR62266-42-6-2 (IR62266), a high-yielding indica ecotype and Norungan, a landrace from TPE, was used to map QTLs for yield and its components under drought predominant in TPE. A large effect yield QTL observed under drought stress in TPE was consistent across two years with a phenotypic variation of 31.3% and 37.9% and additive effect of 629.2 and 424.9 kg/ha. Further, this region was fine-mapped to 94.0 kb with positive effect on grain yield under stress.
机译:干旱胁迫是一个严重的制约因素,特别是在雨育稻米生产中,通过基于胁迫下产量的选择进行耐旱育种虽然有效,但进展缓慢。在干旱胁迫的环境目标种群(TPE)中占主导地位的干旱胁迫下,为产量及其组成部分绘制数量性状基因座(QTL)将有助于克服这一限制。在本研究中,来自高产in型生态型IR62266-42-6-2(IR62266)和来自TPE的地方品种Norungan的143个F-8和F-9重组近交(RI)系的子集用于绘制在TPE中主要干旱的QTLs的产量及其组成部分的图谱。在TPE的干旱胁迫下,观察到的较大产量QTL在两年内保持一致,表型变异为31.3%和37.9%,累加效应为629.2和424.9 kg / ha。此外,该区域被精细映射到94.0 kb,对应力下的谷物产量具有积极影响。

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