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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Hemolytic activity of mycobacterial TlyA (Rv1694) is independent of its rRNA methylation activity
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Hemolytic activity of mycobacterial TlyA (Rv1694) is independent of its rRNA methylation activity

机译：分枝杆菌TlyA（Rv1694）的溶血活性与其rRNA甲基化活性无关

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摘要

In the present study, we show that the mycobacterial TlyA (Rv1694) is a 'moonlighting' protein, while the intracellular form methlylates the 2 '-O-ribose of ribosomal RNA (conferring susceptibility to the second line of the antibiotic capreomycin); the extracellular form is suspected to be a non-conventional membrane damaging entity. The purified Rv1694 can self-assemble into large oligomers upon contact with phagosomal membranes or red blood cells. Rv1694 has the well-conserved K-69-D-154-K-182-E-238 residues that are essential for site-specific methyltransferase activity. Using the Escherichia coli model system, we show that mutation of any of these residues can result in resistance to capreomycin, while the mutated TlyA can reach the cell wall of the bacterium. The E. coli/TlyA system described here would serve as a simple and reliable model for studying the mechanism of export of TlyA and its homologues, their topology on the cell wall and the nature of contacts it must establish with target phagosomal membranes for their susceptibility.
机译：在本研究中，我们表明分枝杆菌TlyA（Rv1694）是一种“月光下的”蛋白，而细胞内形式则甲基化了核糖体RNA的2'-O-核糖（赋予对抗生素capreomycin的第二线敏感性）。细胞外形式被怀疑是非常规的膜破坏实体。纯化的Rv1694与吞噬体膜或红细胞接触后可自组装成大的寡聚体。 Rv1694具有保守的K-69-D-154-K-182-E-238残基，这些残基对于位点特异性甲基转移酶活性至关重要。使用大肠埃希氏菌模型系统，我们表明这些残基中任何一个的突变都可以导致对毛霉素的抗性，而突变的TlyA可以到达细菌的细胞壁。此处描述的大肠杆菌/ TlyA系统将用作研究TlyA及其同源物输出机制，其在细胞壁上的拓扑结构以及必须与目标吞噬体膜建立接触性的简单可靠模型。

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