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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >In vitro production of camptothecin (an anticancer drug) through albino plants of Ophiorrhiza rugosa var. decumbens.
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In vitro production of camptothecin (an anticancer drug) through albino plants of Ophiorrhiza rugosa var. decumbens.

机译：通过皱叶青霉的白化植物体外生产喜树碱（一种抗癌药）。角质。

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摘要

An experiment was conducted for the in vitro propagation of spontaneously originated albino plant of Ophiorrhiza rugosa var. decumbens, and the analysis of camptothecin (CPT) yield in albino plants with respect to benzyladenine concentrations (2-6 mg/litre) compared with the green (normal) plants in vitro. The production of secondary metabolites by suspension cultured, induction of untransformed and transformed roots, elicitation, immobilization and induction of variants with high productivity of the compounds were emphasized. The frequency of shoots developed from leaf explants cultured on MS medium with different benzyladenine concentrations was similar in albino and green plants. In both cases, MS medium supplemented with 4 mg benzyladenine/litre developed the highest number of shoots. Shoot buds from the explants were initiated within 10-15 days. After 25 days, the leaves turned reddish. Above the optimum benzyladenine level (4 mg/litre), there was a decrease in the number of shoots. Although the albino plants were grown under light, there was no development of the chlorophyll pigment. CPT yield showed significant difference in albinos and normal plants grown on medium with similar benzyladenine concentrations. Production of CPT increased with respect to benzyladenine concentrations. The highest CPT level was recorded from normal and albino plants when the medium was supplemented with 6 mg benzyladenine/litre and CPT yield was 0.311 and 1.04 mg/g dry weight, respectively. In the case of optimum shoot multiplication medium, both in normal and albino plants, although there was an increase in biomass (16.8+or-1.05 and 4.8+or-0.25 g, respectively), CPT yield was less (0.037 and 0.558 mg/g dry weight, respectively). Sucrose has significant effect on CPT production. Albino plants in MS medium with 4 mg benzyladenine/litre and 3% sucrose produced higher amounts of CPT (0.558 mg/g dry weight) compared to 4% (0.207 mg/g dry weight) and 5% (0.198 mg/g dry weight) sucrose.
机译：进行了自发自发的Ophiorrhiza rugosa var的白化植物的体外繁殖实验。与天然绿色（正常）植物相比，白化植物中喜树碱的喜树碱（CPT）产量分析（相对于苄腺嘌呤浓度（2-6 mg / L））。强调了悬浮培养产生次级代谢产物，诱导未转化和转化的根，诱导，固定化和诱导具有高产率化合物的变体。在白化病和绿色植物中，在不同苄基腺嘌呤浓度的MS培养基上培养的叶片外植体发育出的芽的频率相似。在这两种情况下，补充4 mg苄腺嘌呤/升的MS培养基均能产生最高的芽数。外植体的芽芽在10-15天内开始。 25天后，叶子变红。高于最佳苄基腺嘌呤水平（4 mg / L），枝条数减少。尽管白化病植物在光照下生长，但叶绿素色素没有发育。在具有相似苄基腺嘌呤浓度的培养基上种植的白化病和正常植物中，CPT产量显示出显着差异。 CPT的产生相对于苄腺嘌呤浓度增加。当向培养基中补充6 mg苄基腺嘌呤/升，CPT产量分别为干重0.311和1.04 mg / g时，正常和白化植物的CPT最高记录。在正常和白化植物中，使用最佳芽繁殖培养基的情况下，尽管生物量有所增加（分别为16.8+或-1.05和4.8+或-0.25 g），但CPT产量却较低（0.037和0.558 mg /克干重）。蔗糖对CPT的产生有重要影响。与4％（0.207 mg / g干重）和5％（0.198 mg / g干重）相比，在4 mg苄基腺嘌呤/升和3％蔗糖的MS培养基中的白化植物产生的CPT量更高（0.558 mg / g干重） ）蔗糖。

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