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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Molecular characterization of Piper nigrum L. cultivars using RAPD markers
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Molecular characterization of Piper nigrum L. cultivars using RAPD markers

机译：利用RAPD标记对黑胡椒进行分子鉴定

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摘要

Black pepper, Piper nigrum L., often referred to as the 'king of spices' is the most important spice in the world. The pepper of commerce is the dried, mature berries of P. nigrum. Indian black pepper is known for its quality and fetches premium price in the market. Majority of the present-day Indian cultivars, numbering about 100, are land races representing direct introduction from the wild. Advanced cultivars have been derived mostly by clonal selection from land races, though a few are of hybrid origin. As India is the primary centre of diversity of P. nigrum, the indigenous genetic resources are reservoirs of useful genes for plant improvement programmes. Unambiguous characterization of cultivars and selected germplasm is an urgent requirement in tune with the globalization of agriculture. Molecular markers offer means of identifying cultivars with much greater reliability than the morphological traits which are governed by complex genetic interactions. Of the several PCR-based techniques, random amplified polymorphic DNA (RAPD) technique offers a simple and economical means for rapid identification of a large number of accessions. The information obtained through germplasm characterization using RAPD is extensively used for the identification of germplasm, screening of duplicates, assessing genetic diversity and monitorlng the genetic stability of conserved germplasm. In the genus Piper, the technique has been successfully utilized in identifying somaclonal variants of Piper longum L. The present study is a report on the DNA fingerprinting of P. nigrum cultivars.
机译：黑胡椒，Piper nigrum L.，通常被称为“香料之王”，是世界上最重要的香料。商业上的胡椒是黑干的成熟浆果。印度黑胡椒以其质量着称，并在市场上获得高价。目前，印度的大多数品种大约有100种，它们是代表从野外直接引进的陆地种族。先进品种主要是通过陆生物种的克隆选择获得的，尽管少数品种是杂种。由于印度是黑斑病菌多样性的主要中心，因此土著遗传资源是植物改良计划的有用基因库。随着农业全球化的发展，对品种和精选种质进行明确的鉴定是迫切的要求。分子标记提供了鉴定品种的方法，其可靠性远高于受复杂遗传相互作用控制的形态特征。在几种基于PCR的技术中，随机扩增多态性DNA（RAPD）技术为快速鉴定大量的种质提供了一种简单且经济的方法。通过使用RAPD进行种质鉴定获得的信息被广泛用于种质鉴定，重复筛查，评估遗传多样性和监测保守种质的遗传稳定性。在Piper属中，该技术已成功地用于鉴定Piper longum L的体细胞克隆变异体。本研究是有关黑假单胞菌（P. nigrum）品种DNA指纹图谱的报道。

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