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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Agrobacterium rhizogenes-mediated transformation of Witloof chicory - Invitro shoot regeneration and induction of flowering
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Agrobacterium rhizogenes-mediated transformation of Witloof chicory - Invitro shoot regeneration and induction of flowering

机译：发根农杆菌介导的菊苣菊苣的转化-体外芽再生和开花诱导

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摘要

Stable transformation and expression of transgene was achieved in Witloof chicory (Cichorium intybus L. cv, Lucknow local) using Agrobacterium rhizogenes-mediated system. Transformation frequencies varied with use of different types of strains of A. rhizogenes, concentration of ii. rhizogenes and age of explants, The transfer of rolA gene into chicory genome was confirmed by PCR analyses. The supplementation of putrescine at 40 mM level with 2'-3'-O-isopropylidene adenosine (2-iP) (2 mg l(-1)) and, gibberellic acid (GA(3)) (0.5 mg l(-1)) to MS medium resulted in increased shoot multiplication and shoot growth in untransformed plants compared to the transformed plants of C, intybus. Putrescine at 40 mM level also resulted in flowering in both the systems on the 28th day, in untransformed and transformed plants. The supplementation of AgNO3 at 40 muM level to the untransformed axillary buds of C, intybus on MS media with 2-iP (2 mg l(-1)) and GA(3) (0.5 mg l(-1)) resulted in higher shoot multiplication (36.9 +/- 2.63 shoots per culture) and higher growth of shoots (7.82 +/- 0.76 cm) in comparison to transformed ones for shoot multiplication (11.6 +/- 0.89 shoots per culture) and growth of shoots (3.2 +/- 0.24 cm), Moreover, 40 muM AgNO3 treated cultures showed in vitro flowering on the 28th day in both the systems. The information generated in terms of transformation protocols, in vitro morphogenesis including flowering will make genetic transformation easier for chicory crop improvement.
机译：使用发根农杆菌介导的系统，在Witloof菊苣（菊苣（Cichorium intybus L.cv，Lucknow local））中实现了稳定的转基因转化和表达。转化频率因使用不同类型的发根农杆菌菌株，ii的浓度而变化。发根和外植体的年龄，通过PCR分析证实了rolA基因向菊苣基因组的转移。在40 mM浓度的腐胺中添加2'-3'-O-异亚丙基腺苷（2-iP）（2 mg l（-1））和赤霉素（GA（3））（0.5 mg l（-1 ））到MS培养基中，与转化的C. intybus植物相比，在未转化的植物中增加了芽的繁殖和芽的生长。在第28天，未转化和转化的植物中40 mM水平的腐胺也导致两个系统开花。在MS培养基上以2-iP（2 mg l（-1））和GA（3）（0.5 mg l（-1））将40 NOM AgNO3补充到未转化的C，线虫的未转化腋芽中的结果更高与转化后的芽繁殖（每个培养物11.6 +/- 0.89个芽）和芽的生长（3.2 + +）相比，芽繁殖（每个培养物36.9 +/- 2.63个芽）和更高的芽生长（7.82 +/- 0.76 cm） （-0.24cm），此外，在两种系统中，在第28天，经40μMAgNO 3处理的培养物均显示出体外开花。就转化方案，包括开花在内的体外形态发生而言，产生的信息将使菊苣作物改良的遗传转化更加容易。

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