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Development of an optical tweezer combined with micromanipulation for DNA and protein nanobioscience

机译:结合显微操作的光学镊子的开发,用于DNA和蛋白质纳米生物科学

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We have constructed an optical tweezer using two lasers (830 nm and 1064 nm) combined with micropipette manipulation having sub-pN force sensitivity. Sample position is controlled within nanometer accuracy using XYZ piezo-electric stage. The position of the bead in the trap is monitored using single particle laser backscattering technique. The instrument is automated to operate in constant force, constant velocity or constant position measurement. We present data on single DNA force-extension, dynamics of DNA integration on membranes and optically trapped bead-cell interactions. A quantitative analysis of single DNA and protein mechanics, assembly and dynamics opens up new possibilities in nanobioscience.
机译:我们使用两个激光(830 nm和1064 nm)结合具有亚pN力敏感度的微量移液器来构造光学镊子。使用XYZ压电平台将样品位置控制在纳米精度内。使用单粒子激光反向散射技术可以监控陷阱中珠子的位置。该仪器可自动进行恒定力,恒定速度或恒定位置测量。我们提供有关单个DNA力扩展,膜上DNA整合动力学和光学捕获的珠细胞相互作用的数据。对单个DNA和蛋白质的力学,组装和动力学进行定量分析,为纳米生物科学开辟了新的可能性。

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