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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Genetic diversity within and among populations of the Indian short-nosed fruit bat, Cynopterus sphinx assessed through RAPD analysis
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Genetic diversity within and among populations of the Indian short-nosed fruit bat, Cynopterus sphinx assessed through RAPD analysis

机译：通过RAPD分析评估印度短鼻果蝠Cynopterus sphinx种群内部和种群之间的遗传多样性

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摘要

Genetic diversity was studied among 40 different zone samples in the Indian short-nosed fruit bat, Cynopterus sphinx. Samples were collected from 127 locations in different districts of southern Tamil Nadu, India. Polymorphism at molecular level was studied by random amplified polymorphic DNA marker technique. Polymerase chain reaction (PCR) with 30 arbitrary decamer oligonucleotide primers was applied to the 40 zone samples. Out of 30 random primers screened, 22 gave reproducible polymorphic bands. PCR product of bat genome revealed a total of 234 bands, out of which 171 were found to be polymorphic. The dendrogram displaying the relative genetic similarities between the zones ranged from 0.42 to 0.92. Principal coordinates analysis revealed that the first two components accounted for 51.4% of the total variation. The results show that considerable genetic diversity was observed in this species from different zonal populations, possibly due to complete dispersal of juveniles of both sexes from their natal groups and gene flow between the zones. The results obtained from this study suggest not only a predictive framework for future studies, but also the use of genetic data in the management and meaningful conservation of this species.
机译：在印度短鼻果蝠Cynopterus sphinx中研究了40个不同区域样本的遗传多样性。从印度泰米尔纳德邦南部不同地区的127个地点收集了样本。通过随机扩增多态性DNA标记技术研究了分子水平的多态性。将具有30个任意十聚体寡核苷酸引物的聚合酶链反应（PCR）应用于40个区域样品。在筛选的30个随机引物中，有22个产生了可再现的多态性条带。蝙蝠基因组的PCR产物显示总共234条带，其中171条被发现是多态的。显示区域之间相对遗传相似性的树状图的范围为0.42至0.92。主坐标分析显示，前两个分量占总变异的51.4％。结果表明，在该物种中，来自不同地带种群的遗传多样性非常可观，这可能是由于两性幼体从其出生群体中完全散布以及区域之间的基因流动所致。从这项研究中获得的结果不仅为今后的研究提供了预测框架，而且还提出了利用遗传数据管理和保护该物种的信息。

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