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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Cryopreservation of subgroup Monthan (ABB) of Indian cooking banana (Musa spp.) germplasm.
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Cryopreservation of subgroup Monthan (ABB) of Indian cooking banana (Musa spp.) germplasm.

机译：冷冻保存印度烹饪香蕉（Musa spp。）种质的Monthan（ABB）亚组。

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A study was conducted to develop an effective and practical cryopreservation protocol for Indian cooking bananas (ABB subgroup) by determining the optimal duration of treatment of sucrose (in preculture medium) and PVS2 to the explants for maximum post-thaw recovery of functional plants. Cultivar Kallu Monthan was used for the optimization of sucrose preculture duration. White meristematic clumps containing 12-20 meristematic domes were excised and transferred onto preculture medium with M1 medium constituents, but with 0.4 M sucrose instead of 0.09 M sucrose. Dictated by the results of optimum sucrose concentration for preculture treatment, optimization of PVS2 treatment was done for cultivars Kallu Monthan, Pidi Monthan and Sommrani Monthan. The sucrose-precultured meristems were transferred to cryovials with 1.5 ml loading solution (LS) for 20 minutes at room temperature, and later LS was removed and 1.5 ml PVS2 was added. The meristems were exposed to PVS2 for 30, 60, 90, 120 and 150 minutes at 0 degrees C. After PVS2 treatment, cryovials with 7-10 clumps were plunged into liquid nitrogen (LN) and cryostored for at least 1 h. After 4-6 weeks of cryopreservation, meristematic clumps exhibited four types of responses: (1) remained pale or white and did not show any further change; (2) turned partially or completely black; (3) formed non-morphogenic callus; and (4) regenerated shoot buds/shoots. For LN-treated explants, 2-week preculture on M1 medium (0.4 M sucrose) was optimum and gave significantly higher post-thaw shoot regeneration (35.3%). The cryopreservation method was effective and has the following advantages: easy operation, less expenditure on laboratory equipment, short processing time, and useful for long-term conservation of banana germplasm.
机译：通过确定蔗糖（在预培养基中）和PVS2处理外植体的最佳处理时间，以最大程度地解冻功能植物，为印度烹饪香蕉（ABB亚组）开发了一种有效且实用的冷冻保存方案。品种Kallu Monthan用于优化蔗糖的预培养时间。切下包含12-20个分生圆顶的白色分生团块，并将其转移到具有M1培养基成分但具有0.4M蔗糖而不是0.09M蔗糖的预培养基上。根据用于预培养处理的最佳蔗糖浓度的结果，对品种Kallu Monthan，Pidi Monthan和Sommrani Monthan的PVS2处理进行了优化。在室温下，将蔗糖预培养的分生组织转移至带有1.5 ml上样溶液（LS）的冷冻管中20分钟，然后取出LS，并添加1.5 ml PVS2。将分生组织在0摄氏度下暴露于PVS2 30、60、90、120和150分钟。PVS2处理后，将具有7-10个团块的冷冻小管插入液氮（LN）中并冷冻至少1 h。冷冻保存4-6周后，分生性团块表现出四种类型的反应：（1）保持淡淡或白色，没有任何进一步的变化; （2）部分或完全变黑; （3）形成的非形态发生的愈伤组织; （4）再生芽/芽。对于经LN处理的外植体，最理想的是在M1培养基（0.4 M蔗糖）上进行2周的预培养，解冻后的芽再生显着更高（35.3％）。冷冻保存方法是有效的，具有以下优点：操作简便，实验室设备费用少，处理时间短，可用于香蕉种质的长期保存。

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