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Time and dose-dependent antimicrobial potential of Ag nanoparticles synthesized by top-down approach

机译:自上而下合成的银纳米颗粒的时间和剂量依赖性抗菌潜能

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摘要

Silver nanoparticles are known to be good antibiotic agents. In this study, silver (Ag) nanoparticles (similar to 6 nm) were synthesized using electro-exploding wire (EEW) technique. Antibacterial action of Ag nanoparticles was studied both in liquidand solid phase using colony-forming unit (CFU) detection. Time and dose-dependent study of Ag nanoparticles shows that the effectiveness of particles increases with increasing particle dose and treatment time. This effect was dose-dependent and more pronounced against Gram-negative bacteria compared to Gram-positive bacteria. Transmission electron microscopy result shows particle binding with bacterial cell membrane. Membrane potential assay and cytoplasm diffusion assay show the effectiveness of Ag nanoparticle used in this study.
机译:已知银纳米颗粒是良好的抗生素剂。在这项研究中,使用电爆炸丝(EEW)技术合成了银(Ag)纳米粒子(类似于6 nm)。使用集落形成单元(CFU)检测研究了液相和固相中Ag纳米颗粒的抗菌作用。对银纳米颗粒的时间和剂量依赖性研究表明,颗粒的有效性随着颗粒剂量和处理时间的增加而增加。与革兰氏阳性菌相比,这种作用是剂量依赖性的,并且对革兰氏阴性菌更明显。透射电子显微镜结果显示颗粒与细菌细胞膜结合。膜电位测定法和细胞质扩散测定法证明了本研究中使用的银纳米粒子的有效性。

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