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首页> 外文期刊>Talanta: The International Journal of Pure and Applied Analytical Chemistry >A microfluidic platform integrating paper adsorption-based sample clean-up and voltage-assisted liquid desorption electrospray ionization mass spectrometry for biological sample analysis
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A microfluidic platform integrating paper adsorption-based sample clean-up and voltage-assisted liquid desorption electrospray ionization mass spectrometry for biological sample analysis

机译：基于纸吸附的样品清理和电压辅助液体解吸电喷雾电离质谱的微流体平台进行生物样本分析

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摘要

Clinical application of direct sampling electrospray ionization mass spectrometry (ESI-MS) remains limited due to problems associated with very "dirty" sample matrices. Herein we report on a microfluidic platform that allows direct mass spectrometric analysis of serum samples of microliter sizes. The platform integrates in-line paper adsorption-based sample clean-up and voltage assisted liquid desorption ESI-MS/MS (VAL DESI-MS/MS) to detect multiple targeted compounds of clinical interest. Adenosine monophosphate (AMP), adenosine diphosphate (ADP), and adenosine triphosphate (ATP) were selected as model analytes. Simultaneous quantification of these compounds in human serum samples was demonstrated. For all the three compounds, linear calibration curves were obtained in a concentration range from 0.20 to 20.0 mu mol/L with r(2) values >= 0.996. Limits of detection were 0.019, 0.015, and 0.011 mu mol/L for AMP, ADP, and ATP, respectively. Recovery was found in the range from 96.5% to 103.5% at spiking concentrations of 0.25 and 2.50 mu mol/L. The results indicate that the proposed microfluidic mass spectrometric platform is robust and effective. It may have a potential in clinical analysis.
机译：直接采样电喷雾电离质谱（ESI-MS）的临床应用由于与非常“脏”样品基质相关的问题，仍然有限。在此，我们报告了一种微流体平台，允许直接质谱分析微升尺寸的血清样本。该平台整合在线纸吸附的样品清理和电压辅助液体解吸ESI-MS / MS（Val Desi-MS / MS），以检测多种临床兴趣的靶向化合​​物。选择腺苷二磷酸（AMP），腺苷二磷酸（ADP）和三磷酸三磷酸（ATP）作为模型分析物。证明了这些化合物在人血清样品中的同时定量。对于所有三种化合物，用R（2）值> = 0.996的浓度范围为0.20至20.0μmmol/ l的线性校准曲线。检测限为0.019,0.015和0.011μmmol/ l用于AMP，ADP和ATP。在0.25和2.50μmmol/ l的尖峰浓度下，恢复的恢复率为96.5％至103.5％。结果表明，所提出的微流体质谱平台具有稳健且有效。它可能具有临床分析的潜力。

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