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首页> 外文期刊>Talanta: The International Journal of Pure and Applied Analytical Chemistry >Spectrophotometric system based on a device created by 3D printing for the accommodation of a webcam chamber as a detection system
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Spectrophotometric system based on a device created by 3D printing for the accommodation of a webcam chamber as a detection system

机译：基于通过3D打印创建的装置的分光光度法系统，用于将网络摄像头室的容纳作为检测系统

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摘要

The development of a simple and economical spectrophotometric system based on the use of a device created by 3D printing and the electronics necessary to control the intensity of the radiation source was described. The measurements are made with a low-cost digital webcam. The entire system is only powered through the USB outputs of a computer, which makes the portable and really practical system for the measurements in the field. This method was applied to determine iron (II) in waters using o-phenanthroline as chromogenic reagent giving a red complex, and also to hypochlorite determination using tetramethylbenzidine as the reagent providing a yellow color. The calibration curves were built using a mathematical algorithm making a RGB deconvolution. The intense of colors obtained from a webcam in each concentration of analyte had a relationship with the absorbance values. In order to confirm the accuracy and precision of this method, a traditional spectrophotometer was used for validation.
机译：描述了基于使用由3D打印产生的装置的简单和经济的分光光度系统的开发和控制辐射源所需的电子设备。测量由低成本的数字网络摄像头进行。整个系统仅通过计算机的USB输出供电，这使得便携式和真正实用的系统用于该字段中的测量值。应用该方法以使用O-菲咯啉作为发色试剂的水中测定铁（II），得到红色复合物，以及使用四甲基苯胺作为提供黄色的试剂的次氯酸盐测定。使用制作RGB折块的数学算法建立校准曲线。在每个浓度的分析物中从摄像头中获得的颜色强烈与吸光度值的关系。为了确认该方法的准确性和精度，使用传统的分光光度计进行验证。

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