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Mining functional information from cereal genomes - the utility of expressed sequence tags [Review]

机译:从谷物基因组中挖掘功能信息-表达的序列标签的实用性[综述]

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摘要

The vast number of expressed sequence tags (ESTs) generated from cereals with large genome size is an important complement method to the whole genome sequencing projects. As technology-driven revolution sweeps through, we are submerged in an avalanche of new information about genes and their function. To accomplish functional roles to the available ESTs, proper annotation of EST data is crucial, which could be achieved through the employment of tools of bioinformatics; generated in the recent past. In this review, the critical steps involved in EST-based gene discovery and the employment of available web-base bioinformatic tools for annotation of ESTs is discussed. The current status of application of E clones in the development of molecular markers in cereal species,and utilizing ESTs as a resource for the construction of arrays is summarized as well. We also focus on large-scale gene expression data analysis methods and the challenges for computational biologists to. extract functional information from such large-scale gene expression data.
机译:从基因组较大的谷物中产生的大量表达序列标签(EST)是整个基因组测序项目的重要补充方法。随着技术驱动的革命席卷而来,我们陷入了关于基因及其功能的大量新信息。为了发挥现有EST的功能作用,正确注释EST数据至关重要,这可以通过使用生物信息学工具来实现;最近产生的。在这篇综述中,讨论了基于EST的基因发现中涉及的关键步骤,以及使用可用的基于Web的生物信息学工具注释EST的关键步骤。总结了E克隆在谷物分子标记开发中的应用现状,以及利用ESTs作为阵列构建资源的现状。我们还专注于大规模基因表达数据分析方法以及计算生物学家所面临的挑战。从此类大规模基因表达数据中提取功能信息。

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