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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Prevalence of white spot syndrome virus in wild crustaceans along the coast of India
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Prevalence of white spot syndrome virus in wild crustaceans along the coast of India

机译：印度沿海野生甲壳动物中白斑综合症病毒的流行

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Crustacean samples comprising of shrimps, crabs and squilla were collected from various landing centres and local fish markets of Kolkata, Chennai, Thiruvananthapuram, Mangalore and Mumbai along the east coast and west coast of India. Nested polymerase chain reaction (PCR) was employed for screening the samples for white spot syndrome virus (WSSV). A total of 89 crustacean samples were examined, which comprised of 40 shrimps, 36 crabs and 13 squilla samples. The results indicate that the wild-caught marine shrimps such as Penaeus monodon, Heterocarpus sp., Aristeus sp., Parapenaeopsis stylifera, Metapenaeus dobsoni, M. elegans and Squilla sp. carry WSSV. This virus could be detected in apparently healthy crabs such as Scylla serrata, Portunus sanguinolentus, P. pelagicus, Calappa lophos, Charybdis hoplites, C. lucifera, C. cruciata and Pseudograpsus intermedius. The virus was also found to be present in the freshwater prawn Macrobrachium rosenbergii, collected from the fish market. Sensitivity of detection of WSSV by PCR improved greatly, with PCR primers yielding amplicons of smaller size. Marine shrimps such as Heterocarpus sp., Aristeus sp., Metapenaeus elegans and crabs such as C. hoplites are being reported as carriers of WSSV. [References: 29]
机译：沿印度东海岸和西海岸从加尔各答，金奈，蒂鲁文南特布勒姆，芒格洛尔和孟买的各个登陆中心和当地鱼市场收集了甲壳类动物样品，包括虾，蟹和金枪鱼。采用巢式聚合酶链反应（PCR）筛选样本中的白斑综合症病毒（WSSV）。总共检查了89个甲壳动物样品，其中包括40只虾，36个螃蟹和13个Squilla样品。结果表明，野生捕捞的海虾，如斑节对虾，杂果类，阿里斯多斯类，气生副对虾，Metapenaeus dobsoni，线虫和鱿鱼。携带WSSV。可以在表面健康的螃蟹中检测到该病毒，这些螃蟹包括锯缘青蟹，Portunus sanguinolentus，P。pelagicus，Calappa lophos，Charybdis hoplites，C。lucifera，C。cruciata和中间假单胞菌。该病毒还被发现存在于从鱼类市场收集的淡水虾罗氏沼虾中。 PCR检测WSSV的灵敏度大大提高，PCR引物可产生较小尺寸的扩增子。据报道，WSSV的携带者有海虾，如杂果树属，亚里士多斯属，秀丽线虫和蟹，如C. Hoplites。 [参考：29]

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