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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Production and properties of alkaline xylanases from Bacillus sp. isolated from sugarcane fields
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Production and properties of alkaline xylanases from Bacillus sp. isolated from sugarcane fields

机译：芽孢杆菌属碱性木聚糖酶的生产和性质。从甘蔗田中分离

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摘要

Xylanolytic bacteria were isolated on oat spelt xylan agar medium and screened by the xylanolysis method. The three bacterial strains isolated from sugarcane fields, St sub(A), St sub(B) and St sub(c) were identified as Bacillus sp. based on morphological, biochemical and physiological characters. Some properties of alkaline xylanases produced by the three strains of Bacillus sp. were studied. Optimal pH and temperature were 9.0 and 55 degree C for strain St sub(A) and 9.0 and 50 degree C for strain St sub(B) and 8.0 and 55 degree C for strain St sub(c) respectively. The enzymes were stable between 30 and 50 degree C and xylanase activity remained up to 2 h for the enzymes of the three strains. Effect of various carbon sources on xylanase production was studied. Enzyme activity was stimulated by beta -mercaptoethanol and dithiothreitol, and decreased by isopropanol. These properties qualify the enzyme to be novel and exhibit favourable potential for application to bleaching in the paper and pulp industry.
机译：在燕麦拼写的木聚糖琼脂培养基上分离木聚糖分解细菌，并通过木聚糖分解方法进行筛选。从甘蔗田分离出的三个细菌菌株，St sub（A），St sub（B）和St sub（c）被鉴定为芽孢杆菌。根据形态，生化和生理特征。三种芽孢杆菌属菌株产生的碱性木聚糖酶的一些特性。被研究了。 St Sub（A）菌株的最佳pH和温度分别为9.0和55摄氏度，St Sub（B）菌株的最佳pH和温度分别为9.0和50摄氏度，St Sub（c）菌株的最佳pH和温度分别为8.0和55摄氏度。该酶在30至50摄氏度之间稳定，三种菌株的酶的木聚糖酶活性保持长达2小时。研究了各种碳源对木聚糖酶生产的影响。 β-巯基乙醇和二硫苏糖醇刺激酶的活性，异丙醇降低酶的活性。这些特性使该酶具有新颖性，并显示出在造纸和纸浆工业中用于漂白的有利潜力。

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