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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Online capillary electrophoresis – mass spectrometry analysis of histatin-5 and its degradation products
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Online capillary electrophoresis – mass spectrometry analysis of histatin-5 and its degradation products

机译：组织素-5及其降解产物的在线毛细管电泳 - 质谱分析及其降解产物

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摘要

Histatin-5 (Hst-5) is a human salivary peptide with antibacterial and antifungal activities. Thorough characterization and reliable quantification of Hst-5 and its degradation products are essential for understanding the Hst-5 degradation pathway. Due to the highly basic and strong cationic nature of the Hst-5 peptide, the quantitative analysis of Hst-5 and its degradation forms by online mass spectrometry remains challenging. Here, we adopt a recently developed electrokinetically pumped sheath liquid capillary electrophoresis – mass spectrometry (CE-MS) coupling technology, and successfully apply it for the analysis of Hst-5 and its degradation products. Our CE-MS method is demonstrated to be robust and quantitative. This novel analytical platform is reproducible and free of sample carryover. The efficacy of this method is demonstrated with a kinetic study of Hst-5 degradation by Sap9, a secreted aspartic peptidase. Our work demonstrates the potential of online CE-MS as a powerful approach for characterizing highly basic peptides.
机译：组织素-5（HST-5）是一种具有抗菌和抗真菌活性的人唾液肽。 HST-5的彻底表征和可靠的定量和其降解产物对于理解HST-5降解途径至关重要。由于HST-5肽的高度基本和强的阳离子性质，HST-5的定量分析及其在线质谱法形式的定量分析仍然具有挑战性。在这里，我们采用新近开发的电动泵鞘液毛细管电泳 - 质谱（CE-MS）耦合技术，并成功地应用它的HST-5及其降解产物的分析。我们的CE-MS方法被证明是坚固和定量的。该新型分析平台可再现，不含样品携带。通过SAP9的HST-5降解的动力学研究证明了该方法的疗效，分泌的天冬氨酸肽酶。我们的作品展示了在线CE-MS的潜力作为表征高基本肽的强大方法。

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