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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Dynamic single-cell intracellular pH sensing using a SERS-active nanopipette
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Dynamic single-cell intracellular pH sensing using a SERS-active nanopipette

机译：使用SERS-活性纳米纤维液动态单细胞细胞内pH感测

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摘要

Glass nanopipettes have shown promise for applications in single-cell manipulation, analysis, and imaging. In recent years, plasmonic nanopipettes have been developed to enable surface-enhanced Raman spectroscopy (SERS) measurements for single-cell analysis. In this work, we developed a SERSactive nanopipette that can be used to perform long-term and reliable intracellular analysis of single living cells with minimal damage, which is achieved by optimizing the nanopipette geometry and the surface density of the gold nanoparticle (AuNP) layer at the nanopipette tip. To demonstrate its ability in singlecell analysis, we used the nanopipette for intracellular pH sensing. Intracellular pH (pH_i) is vital to cells as it influences cell function and behavior and pathological conditions. The pH sensitivity was realized by simply modifying the AuNP layer with the pH reporter molecule 4-mercaptobenzoic acid. With a response time of less than 5 seconds, the pH sensing range is from 6.0 to 8.0 and the maximum sensitivity is 0.2 pH units. We monitored the pH_i change of individual HeLa and fibroblast cells, triggered by the extracellular pH (pH_e) change. The HeLa cancer cells can better resist pH_e change and adapt to the weak acidic environment. Plasmonic nanopipettes can be further developed to monitor other intracellular biomarkers.
机译：玻璃纳米坯已经显示出在单细胞操纵，分析和成像中的应用。近年来，已经开发了等离子体纳米浦倾屈，以实现表面增强的拉曼光谱（SERS）测量用于单细胞分析。在这项工作中，我们开发了一种具有最小损伤的单级活性细胞的长期和可靠的细胞内分析，其通过优化金纳米颗粒（AUNP）层的表面密度来实现长期和可靠的细胞内分析。通过优化金纳米粒子（AUNP）层的表面密度来实现在纳米纤维尖端。为了证明其在SingleCell分析中的能力，我们使用纳米纤维用于细胞内pH感测。细胞内pH（pH_i）对细胞是至关重要的，因为它会影响细胞功能和行为和病理条件。通过用pH记者分子4-巯基苯甲酸简单地改变AUNP层来实现pH敏感性。响应时间小于5秒，pH感测范围为6.0至8.0，最大灵敏度为0.2 pH单位。我们监测了单个HeLa和成纤维细胞的pH_i变化，由细胞外pH（pH_e）变化引发。 HeLa癌细胞可以更好地抵抗pH_E的变化并适应弱酸环境。可以进一步开发等离子体纳米坯来监测其他细胞内生物标志物。

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