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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Evaluation for regional difference of skin-gas ethanol and sweat rate using alcohol dehydrogenase-mediated fluorometric gasimaging system (sniff-cam)?
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Evaluation for regional difference of skin-gas ethanol and sweat rate using alcohol dehydrogenase-mediated fluorometric gasimaging system (sniff-cam)?

机译：使用醇脱氢酶介导的荧光型燃料系统（Sniff-Cam）对皮肤气体乙醇和汗液速率的区域差异评价？

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Skin gas that contains volatile metabolites (volatilome) is emanated continuously and is thus expected to be suitable for non-invasive monitoring. The aim of this study was to investigate the relationship between the regional difference of sweat rate and skin volatilome distribution to identify the suitable site to monitor metabolisms. In this study, we developed a biofluorometric gas-imaging system (sniff-cam) based on nicotinamide adenine dinucleotide (NAD)-dependent alcohol dehydrogenase (ADH) to visualize transcutaneous ethanol (EtOH) distribution. The EtOH distribution was converted to a fluorescence distribution of reduced NAD with autofluorescence property. First, we optimized the solution volume and concentration of the oxidized NAD, which was a coenzyme of ADH. Owing to the optimization, a two-dimensional distribution of EtOH could be visualized from 0.05-10 ppm with good sensitivity and selectivity. Subsequently, transcutaneous EtOH imaging and measurement of sweat rate were performed at the palm, dorsum of hand, and wrist of participants who consumed alcohol. Transcutaneous EtOH from all skin parts was imaged using the sniff-cam; the concentrations initially increased until 30 min after drinking, followed by a gradual decrease. Although the determined peak EtOH concentrations of typical subjects were approximately 1100 ± 35 ppb (palm), which were higher than 720 ± 18 ppb (dorsum) and 620 ± 13 ppb (wrist), the results of sweat rate suggested that the dorsum of hand and the wrist were appropriate sites. Finally, the sniff-cam could visualize the individual difference of alcohol metabolism capacity originating from aldehyde dehydrogenase phenotype by imaging transcutaneous EtOH.
机译：含有挥发性代谢物（波动组）的皮肤是连续散发的，因此预期适用于非侵入性监测。本研究的目的是调查汗水率和皮肤挥发物分布的区域差异之间的关系，以确定适当的部位以监测代谢。在这项研究中，我们开发了一种基于烟酰胺腺嘌呤二核苷酸（NAD） - 依赖性醇脱氢酶（ADH）的生物荧光测定气体成像系统（Sniff-Cam）以使经皮乙醇（EtOH）分布可视化。将EtOH分布转化为具有自发性性的减少NAD的荧光分布。首先，我们优化了氧化NAD的溶液体积和浓度，这是ADH的辅酶。由于优化，EtOH的二维分布可以从0.05-10ppm可视化，具有良好的灵敏度和选择性。随后，在手掌，手中进行经皮籽籽成像和汗液测量，以及消耗酒精的参与者的手腕。所有皮肤部件的经皮eTOH使用嗅探器成像;浓度最初在饮酒后升至30分钟，然后逐渐减少。虽然所确定的典型受试者的峰EtOH浓度约为1100±35ppb（棕榈），但高于720±18 ppb（背部）和620±13 ppb（手腕），汗水率的结果表明手的背部手腕是合适的网站。最后，通过成像经皮EtOH，嗅探凸轮可视化源自醛脱氢酶表型的醇代谢能力的个体差异。

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