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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Acellular oxidative potential assay for screening of amorphous silica nanoparticles
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Acellular oxidative potential assay for screening of amorphous silica nanoparticles

机译：用于筛选无定形二氧化硅纳米粒子的无细胞氧化潜力测定

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摘要

Silica nanoparticles (SiNPs) are used in a wide range of consumer products, engineering and medical applications, with likelihood of human exposure and potential health concerns. It is essential to generate toxicity information on SiNP forms and associated physicochemical determinants to conduct risk assessment on these new materials. To address this knowledge gap, we screened a panel of custom synthesized, well-characterized amorphous SiNPs pristine and surface-modified (–C3-COOH, –C11-COOH, –NH_2, –PEG) of 5 different sizes: (15, 30, 50, 75, 100 nm) for their oxidative potential using an acellular assay. The assay is based on oxidation of dithiothreitol (DTT) by reactive oxygen species and can serve as a surrogate test for oxidative stress. These materials were characterized for size distribution, aggregation, crystallinity, surface area, surface modification, surface charge and metal content. Tests for association between oxidative potential of SiNPs and their physicochemical properties were carried out using analysis of variance and correlation analyses. These test results suggest that the size of amorphous SiNPs influenced their oxidative potential irrespective of the surface modification, with 15 nm exhibiting relatively higher oxidative potential compared to the other sizes. Furthermore, SiNP surface area, surface modification and agglomeration in solution also appeared to affect oxidative potential of these SiNPs. These findings indicate that physicochemical properties are critical in influencing the oxidative behaviour of amorphous SiNPs, with potential to trigger cellular oxidative stress and thus toxicity, when exposed. This information advances our understanding of potential toxicities of these amorphous SiNPs and supports risk assessment efforts and the design of safer forms of silica nanomaterials.
机译：二氧化硅纳米粒子（SINPS）用于广泛的消费产品，工程和医疗应用，有可能的人体暴露和潜在的健康问题。必须生成关于SINP形式和相关的物理化学决定簇的毒性信息，对这些新材料进行风险评估。为了解决这一知识差距，我们筛选了一种定制的合成，特征良好的无定形Sinps原始和表面改性（-C3-CoOH，-C11-CoOH，-NH_2，-PEG）的面板，为5种不同的尺寸：（15,30使用细粒测定，50,75,100nm）用于氧化潜力。测定基于反应性氧物质的二硫代噻唑醇（DTT）的氧化，并且可以作为氧化应激的替代试验。这些材料的特征在于尺寸分布，聚集，结晶度，表面积，表面改性，表面电荷和金属含量。使用方差分析进行SINPS的氧化潜力与其物理化学性质之间的测试及其物理化学性能。这些测试结果表明，无定形SINP的尺寸影响其氧化潜力，而不管表面改性如何，与其他尺寸相比，15nm表现出相对较高的氧化潜力。此外，SONP表面积，表面改性和溶液中的附聚也似乎影响了这些SINP的氧化潜力。这些发现表明，物理化学性质在影响无定形SINPS的氧化行为方面至关重要，潜在地触发细胞氧化应激并因此毒性。这些信息推进了我们对这些无定形SINPS的潜在毒性的理解，并支持风险评估努力和硅氧化硅纳米材料的更安全形式的设计。

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