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Productive screening of single aptamers with ddPCR

机译:具有DDPCR的单一适体的生产筛选

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摘要

Antibodies have now been widely used for clinical treatment of a number of tumors. However, there are serious problems associated with antibody therapy, such as potential interactions of antibodies with the immune system as well as long production cycles. Recently, aptamers have been found to function similar to antibodies in terms of affinity and specificity to certain proteins and are attracting much attention for their low immunogenicity, easy chemical synthesis, and efficient penetration into tissues due to their small size. However, how to access high affinity and selectivity aptamers efficiently for further analysis is still open to be resolved. Herein, an aptamer discovery method that combines the continuous flow ddPCR technology with cytometer sorting of beads is reported, such that we have obtained DNA aptamers binding specifically to PD-1 with an affinity of over 60-fold higher than that for the best-reported method.
机译:抗体现在已被广泛用于许多肿瘤的临床治疗。 然而,存在与抗体治疗相关的严重问题,例如抗体与免疫系统的潜在相互作用以及长的生产循环。 最近,已发现适体与某些蛋白质的亲和力和特异性的抗体相似,并且由于它们的小尺寸而吸引了它们的低免疫原性,易化学合成,并且有效地渗透到组织中。 但是,如何在进一步分析中有效地访问高亲和力和选择性Aptamers仍然打开。 据报道,据报道,将连续流动DDPCR技术结合的APTamer发现方法,使得我们已经获得了特异性的DNA适体与PD-1的亲和力高于60倍,比最佳报道的亲和力 方法。

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