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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Comparison of standard and HD FT-IR with multimodal CARS/TPEF/SHG/FLIMS imaging in the detection of the early stage of pulmonary metastasis of murine breast cancer
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Comparison of standard and HD FT-IR with multimodal CARS/TPEF/SHG/FLIMS imaging in the detection of the early stage of pulmonary metastasis of murine breast cancer

机译：用多式联运和TPEF / SHG / SLIMS成像的标准和高清FT-IR比较检测鼠乳腺癌肺转移早期阶段

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摘要

Lungs, due to their high oxygen availability and vascularization, are an ideal environment for cancer cell migration, metastasis and tumour formation. These processes are directly connected with extracellular matrix (ECM) remodelling, resulting from cancer cell infiltration and preparation of the environment suitable for tumour growth. Herein, we compare the potential of fast, label-free and non-destructive methods of Fourier-transform infrared spectroscopy (FT-IR) in standard and high definition (HD) modes with nonlinear coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), twophoton excited fluorescence (TPEF) and a fluorescence lifetime imaging (FLIM) technique for lung metastasis detection. We show their potential in the detection of lung macrometastasis, in which we already observed the ECM remodelling. The CARS image revealed a dense cell fraction typical of ECM remodeling and reduction of the TPEF signal together with an increase of fluorescence lifetime predominantly due to NAD(P)H suggesting metabolic changes in the metastatic foci. FT-IR spectroscopy allowed not only for macrometastasis detection but also their stage definition based mainly on the analysis of proteins, RNA and glycogen fractions. The multimodal approach additionally suggested partial enzymatic degradation of elastin in ECM and collagen remodelling.
机译：由于其高氧可用性和血管化，肺部是癌细胞迁移，转移和肿瘤形成的理想环境。这些方法与细胞外基质（ECM）重塑直接连接，由癌细胞浸润产生并制备适合于肿瘤生长的环境。在此，我们将傅立叶变换红外光谱（FT-IR）的快速，标签和非破坏性方法的潜力进行标准和高清晰度（HD）模式，其中具有非线性相干抗Stokes拉曼散射（汽车），第二谐波生成（SHG），Twophoton激发荧光（TPEF）和肺转移检测的荧光寿命成像（FLIM）技术。我们展示了他们在检测肺癌的潜力，我们已经观察到ECM重塑。汽车图像揭示了典型的ECM重塑和TPEF信号的致密电池级分，以及由于NAD（P）H主要增加荧光寿命，提示转移性焦点的代谢变化。 FT-IR光谱不仅允许用于宏观摩托斯检测，而且还允许其阶段定义主要基于蛋白质，RNA和糖原馏分的分析。多模式方法另外提出了ECM和胶原重塑中弹性蛋白的部分酶促降解。

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