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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Abdominal dropsy disease in major carps of Meghalaya: isolation and characterization of Aeromonas hydrophila.
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Abdominal dropsy disease in major carps of Meghalaya: isolation and characterization of Aeromonas hydrophila.

机译：梅加拉亚邦主要鲤鱼的腹部水肿病：嗜水气单胞菌的分离和鉴定。

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摘要

The biochemical and genetic characteristics of A. hydrophila isolated from catla (Catla catla) and mrigal (Cirrhinus mrigala) with dropsy in Meghalaya, India [date not given], are presented. Symptoms were observed in 5-10% of the fish population, with 5% mortality in fish weighing ~500 g. Degeneration of the lamellar structure of the gills, congested liver, distended intestines and fluid in the abdomen were found during postmortem examination of affected fish. All isolates fermented arabinose, salicin, galactose, lactose, xylose, sucrose, mannose and maltose sugars. Experimental infection of catla and mrigal with 1.5x109 CFU/0.2 ml/fish resulted in the appearance of clinical signs after 72 h followed by death after 4 days in catla and 6-8 days postinfection in mrigal. The isolates were sensitive to gentamicin chlortetracycline; resistant to ampicillin, chloramphenicol and erythromycin and showed varying resistance to cloxacillin. The isolates also showed different polypeptide patterns. Virulence factors including amylase, gelatinase, caseinase, DNAase and beta -haemolysin were also produced by the isolates. Haemolysin and aerolysin genes were amplified using polymerase chain reaction (PCR). These findings confirm the diagnosis of an A. hydrophila outbreak in catla and mrigal cultured in Meghalaya..
机译：介绍了在印度梅加拉亚邦从卡塔拉（Catla catla）和沙棘（Cirrhinus mrigala）分离得到的嗜水链球菌的生化和遗传特性[未提供]。在5-10％的鱼类种群中观察到症状，重约500 g的鱼类死亡率为5％。在对患鱼进行尸检后发现found的层状结构退化，肝脏充血，肠扩张和腹部积液。所有分离物均发酵阿拉伯糖，水杨素，半乳糖，乳糖，木糖，蔗糖，甘露糖和麦芽糖。用1.5x109 CFU / 0.2 ml /鱼的卡塔拉和壁rig进行实验性感染会在72小时后出现临床体征，然后在卡塔拉中感染4天后以及在壁rig感染后6-8天后死亡。分离株对庆大霉素金霉素敏感。对氨苄西林，氯霉素和红霉素有耐药性，对氯沙西林表现出不同的耐药性。分离物还显示出不同的多肽模式。分离物还产生了包括淀粉酶，明胶酶，酪蛋白酶，DNA酶和β-溶血素在内的致病因子。使用聚合酶链反应（PCR）扩增溶血素和气溶素基因。这些发现证实了在梅加拉亚邦（Meghalaya）培养的卡塔拉和海藻中嗜水链球菌暴发的诊断。

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