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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >A novel solution-gated graphene transistor biosensor for ultrasensitive detection of trinucleotide repeats
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A novel solution-gated graphene transistor biosensor for ultrasensitive detection of trinucleotide repeats

机译：一种新型溶液门的石墨烯晶体管生物传感器，用于超敏化酮重复检测

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摘要

A new way to detect GAA trinucleotide repeats (TNRs) based on a solution-gated graphene transistor (SGGT) with high performance was developed. Friedreich’s ataxia (FRDA) is a neurodegenerative disease where the first intron of the frataxin (FXN) gene exhibits an extended GAA repeat region. Herein, a SGGT biosensor was constructed based on G-quadruplex DNAzymes and graphene channels. The DNAzymes quantify the captured target DNA by producing a strong catalytic current signal depending on the peroxidase- like activity. The higher the target DNA quantity captured on the gate electrode is, the higher is the concentration of DNAzymes on the surface of the gate electrode, which generates a high catalytic current. Due to the excellent self-amplifying performance of the transistor, the current signal of the SGGT is several hundreds of times larger than in conventional electrochemistry under identical detection conditions. Moreover, a large current signal can be obtained in the case of a low concentration of H_2O_2 when compared to the case of an enzyme-catalyzed transistor. The SGGT biosensor also exhibits an ultra-low detection limit (32.25 fM), a wide linear range (100 fM–100 nM), and excellent selectivity. The results show that the SGGT biosensor has great potential in the early diagnosis of neurodegenerative diseases.
机译：显影基于具有高性能的溶液门的石墨烯晶体管（SGGT）来检测GaA三核苷酸重复（TNR）的新方法。 Friedreich的共济失调（FRDA）是一种神经变性疾病，其中脱脂蛋白（FXN）基因的第一个内含子表现出扩展的GAA重复区域。在此，基于G-QuadrepledDNazymes和石墨烯通道构建SCGT生物传感器。通过根据过氧化物酶样活性产生强催化电流信号，DNazymes量化捕获的靶DNA。栅电极上捕获的靶DNA量越高，栅电极表面上的dNazymes的浓度越高，产生高催化电流。由于晶体管的优异自放大性能，SGGT的电流信号在相同的检测条件下的传统电化学中的数百倍。此外，与酶催化晶体管的情况相比，在低浓度的H_2O_2的情况下，可以获得大电流信号。 SGGT生物传感器还表现出超低检测限（32.25件FM），宽线性范围（100 FM-100nm）和优异的选择性。结果表明，SGGT生物传感器在早期诊断中具有很大的潜力。

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  • 作者

    Zhiqi Ge; Mingyu Ma; Gang Chang; Meijun Chen; Hanping He; Xiuhua Zhang; Shengfu Wang;

  • 作者单位

    Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules Hubei University No. 368 Youyi Avenue Wuchang Wuhan 430062 China.;

    Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials Hubei Key Laboratory of Polymer Materials Faculty of Materials Science and Engineering Hubei University No. 368 Youyi Avenue Wuchang Wuhan 430062;

    Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials Hubei Key Laboratory of Polymer Materials Faculty of Materials Science and Engineering Hubei University No. 368 Youyi Avenue Wuchang Wuhan 430062;

    Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules Hubei University No. 368 Youyi Avenue Wuchang Wuhan 430062 China.;

    Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules Hubei University No. 368 Youyi Avenue Wuchang Wuhan 430062 China.;

    Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules Hubei University No. 368 Youyi Avenue Wuchang Wuhan 430062 China.;

    Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules Hubei University No. 368 Youyi Avenue Wuchang Wuhan 430062 China.;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分析化学;
  • 关键词

    A novel solution-gated; graphene transistor biosensor; ultrasensitive detection;

    机译：一种新型溶液门;石墨烯晶体管生物传感器;超敏检测;

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