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Direct identification of fish species by surface molecular transferring

机译:通过表面分子转移直接鉴定鱼类

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摘要

With the expansion of the aquatic market and the large quantity of seafood consumption, the issues on safety, traceability and authenticity of seafood are becoming more and more important. Herein, a mass spectrometric method by direct analysis of fish samples was developed for fish authentication. A highthroughput matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) technique was applied to analyze the substances on the fish skin, or the surface molecularly imprinted substances on the surface of muscle tissues using a MALDI-target plate. A multivariate analysis was executed on the obtained mass spectra, and plots of principal component analysis (PCA) for different fish samples were differently clustered in a 95% confidence level. The developed strategy was capable of classifying and identifying fish species. The molecular imprinting method was found with good analytical reproducibility. The strategy enables the distinguishment of fish samples in a quick, efficient and easy mode. It is promising to apply the presently developed strategy for the authentication of seafood and extend the protocol for the detection of other protein food products.
机译:随着水生市场的扩大和大量的海鲜消费,海鲜安全,可追溯性和真实性的问题变得越来越重要。这里,通过对鱼类样品进行直接分析的质谱法用于鱼类认证。施用高渗漏基质辅助激光解吸/电离飞行时间质谱法(MALDI TOF MS)技术,用于使用MALDI-TARGED分析鱼皮肤上的物质,或表面分子在肌肉组织表面上的物质压印物质盘子。在获得的质谱上执行多变量分析,不同鱼类样品的主要成分分析(PCA)的曲线均为95%的置信水平。开发的策略能够进行分类和识别鱼类。发现分子印迹方法具有良好的分析再现性。该策略使鱼类样本能够以快速,高效且简单的模式区分。有希望应用目前开发的海产认证策略,并扩展了检测其他蛋白质食品的方案。

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