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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Molecularly imprinted nanoparticles-based assay (MINA) - detection of leukotrienes and insulin
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Molecularly imprinted nanoparticles-based assay (MINA) - detection of leukotrienes and insulin

机译：基于分子印迹的纳米颗粒的测定（MINA） - 检测白酮和胰岛素

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摘要

A novel molecularly imprinted polymer nanoparticle-based assay (MINA) performed in magnetic microplates was developed as an improved high-quality alternative to existing antibody-based immunoassays. MINA is a generic technology that can be adapted for biomarker detection in biological samples. Herein, we demonstrate the applicability of the MINA assay for the detection of leukotrienes and insulin in biological samples. MINA, used in a competition format, has allowed the detection of LTE4 in urine in a concentration range from 0.45 to 364 pM, with a LOD of 0.73 pM. MINA, used in a competition format, has allowed the detection of insulin in plasma in a concentration range from 25 to 2500 pM, with a LOD of 27 pM. This assay has shown comparable performance for LTE4 and insulin detection to existing chromatographic techniques (LC-MS/MS) and immunoassays in clinically relevant concentrations. The main advantages of this assay are the efficient and low cost fabrication, preparation of synthetic binders without the use of animals, and fewer steps used in the assay protocol as compared to traditional immunoassays.
机译：在磁性微孔板中进行的新型分子印迹聚合物纳米颗粒基测定（MINA）作为现有的基于抗体类免疫测定的改进的高质量替代品。 MINA是一种通用技术，可以适用于生物样品中的生物标志物检测。在此，我们证明了MINA测定对生物样品中白三烯和胰岛素的应用的适用性。 MINA以竞争格式使用，允许在浓度范围内检测尿液中的LTE4，从0.45〜364pm的下午，下午0.73点。 MINA以竞争格式使用，使血浆中胰岛素的浓度范围从25至2500点的血浆检测到27下午27点。该测定表明了LTE4和胰岛素检测的相当性能，以及临床相关浓度的现有色谱技术（LC-MS / MS）和免疫测定。该测定的主要优点是有效且低成本的制造，合成粘合剂的制备而不使用动物，与传统免疫测定相比，测定方案中使用的较少步骤。

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