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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >A potentiometric immunosensor for enterovirus 71 based on bis-MPA-COOH dendrimer-doped AgCl nanospheres with a silver ion-selective electrode
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A potentiometric immunosensor for enterovirus 71 based on bis-MPA-COOH dendrimer-doped AgCl nanospheres with a silver ion-selective electrode

机译：基于BIS-MPA-COOH Dendimer掺杂AgCl纳米的肠道病毒71具有银离子选择性电极的电位免疫传感器

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Herein a new potentiometric immunoassay for the point-of-care detection of enterovirus 71 (EV71) was developed by using a silver (Ag+) ion-selective electrode (ISE). Initially, the carboxylated dendrimer-doped AgCl nanospheres were synthesized by the reverse micelle method. Then the synthesized nanospheres were used to label a polyclonal mouse anti-EV71 antibody via a typical carbodiimide coupling method. The immunoreaction was executed on a monoclonal anti-EV71 antibody-coated microplate by using biofunctional AgCl nanospheres as the detection antibody. With a sandwich-type immunoassay format, the carried AgCl nanospheres could be dissolved in the presence of NH3 center dot H2O, and the released silver ions were determined with an external silver ion-selective electrode. Under optimal conditions, the shift in the potential increased with the increase in the EV71 concentration, in a wide linear range of 0.3-300 ng mL(-1), with a detection limit of 0.058 ng mL(-1). Intra-and inter-assay relative standard deviations with identical batches were less than 4.15% and 6.15%, respectively. By validating the spiked serum samples, our system shows consistency with the enzyme-linked immunosorbent assay (ELISA) kit.
机译：本文通过使用银（Ag +）离子选择性电极（ISE）开发了用于肠道病毒71（EV71）的护理点检测的新电位免疫测定。最初，通过反向胶束方法合成羧化树枝状掺杂的ACCL纳米球。然后，使用合成的纳米球通过典型的碳二亚胺偶联方法标记多克隆小鼠抗EV71抗体。通过使用生物官能AgCl纳米球作为检测抗体，在单克隆抗EV71抗体涂覆的微孔板上执行免疫反应。通过夹层型免疫测定形式，可以在NH3中心点H2O存在下溶解载体AgCl纳米球，并用外部银离子选择性电极测定释放的银离子。在最佳条件下，电位的变化随着EV71浓度的增加而增加，在0.3-300ng mL（-1）的宽线性范围内，检测限为0.058 ng ml（-1）。具有相同批次的测定帧间间相对标准偏差分别小于4.15％和6.15％。通过验证尖刺的血清样品，我们的系统显示与酶联免疫吸附测定（ELISA）试剂盒的一致性。

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