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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Simultaneous monitoring of different vitrification solution components permeating into tissues
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Simultaneous monitoring of different vitrification solution components permeating into tissues

机译：同时监测不同玻璃化溶液组分渗透到组织中的组分

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摘要

Cryopreservation can be used for long-term preservation of tissues and organs. It relies on using complex mixtures of cryoprotective agents (CPAs) to reduce the damaging effects of freezing, but care should be taken to avoid toxic effects of CPAs themselves. In order to rationally design cryopreservation strategies for tissues, it is important to precisely determine permeation kinetics of the protectants that are used to ensure maximum permeation, while minimizing the exposure time and toxicity effects. This is particularly challenging with protectant solutions consisting of multiple components each with different physical properties and diffusing at a different rate. In this study, we show that an attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) setup can be used to simultaneously monitor diffusion of multiple components in a mixture into tissues in real time. Diffusion studies were done with decellularized heart valves using a sucrose-DMSO mixture as well as vitrification solution VS83. To assess diffusion kinetics of different solutes in mixtures, the increase in specific infrared absorbance bands was monitored during diffusion through the tissue. Solute specific wavenumber ranges were selected, and the calculated area was assumed to be proportional to the CPA concentration in the tissue. A diffusion equation based on Fick's second law of diffusion fitted the experimental data quite well, and clear differences in permeation rates were observed among the different mixture components dependent on molecular size and physical properties.
机译：冷冻保存可用于长期保存组织和器官。它依赖于使用密度冷冻剂（CPA）的复杂混合物来降低冻结的破坏性效果，但应注意避免CPA本身的毒性效应。为了理性地设计用于组织的冷冻保存策略，重要的是精确地确定用于确保最大渗透的保护剂的渗透动力学，同时最小化暴露时间和毒性效应。这尤其具有挑战性，保护溶液由具有不同物理性质的多个组分组成，并以不同的速率扩散。在本研究中，我们表明，衰减的总反射 - 傅里叶变换红外光谱（ATR-FTIR）设置可用于同时将混合物中的多个组分的扩散实时地监测到组织中。使用蔗糖-DMSO混合物以及玻璃化溶液Vs83，用脱细胞心阀进行扩散研究。为了评估混合物中不同溶质的扩散动力学，在通过组织的扩散期间监测特异性红外吸光带的增加。选择溶质特异性波数范围，并假设计算的区域与组织中的CPA浓度成比例。基于Fick的第二扩散定律的扩散方程非常好地拟合实验数据，并且在依赖于分子尺寸和物理性质的不同混合物组分中观察到渗透速率的明显差异。

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